Chilled (10°C) and frozen (−15°C) broiler carcasses initially artificially contaminated either with Pseudomonas aeruginosa, Salmonella typhimurium or Serratia marcescens (106cfu/g) were irradiated (Co60) with doses of 0.5, 1.0, 2.5, 5.0 and 10.0 kGy. Ps. aeruginosa was eliminated by doses of 1.0 - 2.5 kGy, S. marcescens by doses of 2.5 - 5.0 kGy and S. typhimurium by a dose of 10 kGy. Characteristic radiation odor increasing with radiation dose and temperature was well removed by heat meat preparation. Radiation resulted in increase of acid and peroxide values and destruction of thiamine (up to 57%/10 kGy) and riboflavin (up to 27%/10 kGy), lower increase of fat indexes and lower destruction of vitamins was observed at lower irradiation temperature. Content of amino acids was not affected by the treatment.

1. Wistar rats were fed for three successive generations on a semi-purified diet, in which the fat was provided by butter, sunflower oil, rapeseed oil or hydrogenated vegetable fat, differing in the content of cis,cis-18:2 and trans-18 : 1 fatty acids. Effects of these fats on the composition of adipose tissue and reproductive performance were studied. Fatty acids were analysed using high-performance liquid chromatography. 2.The fatty acid pattern of adipose tissue was closely related to dietary fat composition and, established in the first generation, did not change significantly in successive generations of rats.3. Hydrogenated fat adversely affected litter size, sperm morphology and regularity of oestrous cycle, and prolonged the period of gestation in experimental animals. Differences observed between the generations were not significant.4. Hydrogenated fat decreased the level of serum testosterone in males, but the differences observed in levels of serum progesterone in females were not apparently related to the dietary tran.r-fatty acids.

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Z������ J., S����� J., K������� K., K�������-K���� A., H���� T., Z���� V., F������� A. (2004): Effects of oxidised dietary cod liver oil on the reproductive functions of Wistar rat. Czech J. Food Sci., 22: 108-120.Weanling Wistar rats, males and females, were fed for 185 days with diets containing 15% of dietary fat in the form of a mixture of lard and partially oxidised cod liver oil. The proportion of cod liver oil in the dietary fat ranged from 0 to 100%, and the content of malondialdehyde from 0.3 to 19.6 mg/kg of the fat used. Animals fed with diets containing higher proportions of oxidised cod liver oil had higher concentrations of malondialdehyde in their livers. Serum lipid levels were lower in animals fed with higher proportions of cod liver oil than in animals fed control diets (milk fat or lard). The lowest concentration of serum lipid was found in the rats fed the diet containing half of its fat as fish oil. Increased intakes of cod liver oil resulted in lower body weight gains, weights of livers, kidneys, and weights of the reproductive organs. The relative weights of livers and kidneys/body weight were higher in the groups with higher intakes of cod liver oil. High intakes of cod liver oil led to a drastically impaired fertility of females, a decreased litter size, a higher postnatal mortality, and an increased incidence of morphologically abnormal spermatozoa in males.

Phenacyl esters of C12-C22 fatty acids were separated on Separon SGX C18 column, using a gradient elution with methanol-acetonitrile-water. The proposed gradient showed better resolution of the critical pairs C18:3-C14:0, C16:1-C20:4, and C16:0-C18:1 than the gradient elution with methanol-water or acetonitrile-water, or than the isocratic elution with methanol-acetonitrile-water. The optimum volume concentration (83%) of the sum of both methanol and acetonitrile was maintained constant for 35 min; in this period the acetonitrile concentration decreased linearly from the initial 42-60% to 0% while the methanol concentration increased from the initial 41-23% to 83% at the same rate. After 35 min the elution was completed with a methanol-water gradient. The whole analysis can be performed within 63 min at a flow rate 1 ml/min.