Effects of dietarytrans-fatty acids on reproductive performance of Wistar rats

Abstract

1. Wistar rats were fed for three successive generations on a semi-purified diet, in which the fat was provided by butter, sunflower oil, rapeseed oil or hydrogenated vegetable fat, differing in the content of cis,cis-18:2 and trans-18 : 1 fatty acids. Effects of these fats on the composition of adipose tissue and reproductive performance were studied. Fatty acids were analysed using high-performance liquid chromatography. 2.The fatty acid pattern of adipose tissue was closely related to dietary fat composition and, established in the first generation, did not change significantly in successive generations of rats.3. Hydrogenated fat adversely affected litter size, sperm morphology and regularity of oestrous cycle, and prolonged the period of gestation in experimental animals. Differences observed between the generations were not significant.4. Hydrogenated fat decreased the level of serum testosterone in males, but the differences observed in levels of serum progesterone in females were not apparently related to the dietary tran.r-fatty acids.

Testosterone-lowering activity of canola and hydrogenated soybean oil in the stroke-prone spontaneously hypertensive rat

“…Early in 1989, Hanis et al (1989) reported that hydrogenated vegetable oil at 5 weight % of the diet adversely affected litter size, sperm morphology, regularity of estrous cycle and serum testosterone level in Wistar rats, compared with sunflower oil and low-erucic acid rapeseed oil. Butterfat also decreased serum testosterone level significantly.…”

High‐energy diets: a threat for male fertility?

“…Indeed, the increased fatty acid content within the testicular milieu contributes to altered testicular lipid metabolism, disrupting the reproductive events that depend on it. The following evidences have supported this hypothesis: (i) male rodents fed with hydrogenated oils instead of nonhydrogenated vegetable oils accumulate trans-fatty acids in the testis (68) and (ii) the accumulated fat in testicular environment resulted in adverse male reproductive outcomes, such as decreased sperm parameters, decreased T levels and even arrested spermatogenesis and testicular degeneration (30,68). In addition, hydrogenated fats and specific fatty acid isomers affect the activity of desaturases, elongases and may disrupt de novo lipogenesis that is highly active in cells of the seminiferous epithelium, particularly in SCs.…”

“…Importantly, consumption of trans ‐fatty acids compromises male fertility, as a close relationship between their consumption and decreased sperm parameters has been consistently reported . As these fats cannot be endogenously synthesized, its excessive consumption contributes to fat accumulation within the testicular environment . Fatty acids accumulate in testicular cells by two distinct processes: passive diffusion through the lipid bilayer and/or protein facilitated transport mediated by CD36 glycoprotein , which is widely expressed in SCs .…”

Determination of Low Level Trans Unsaturation in Fats by Fourier Transform Infrared Spectroscopy

“…Trans unsaturated fatty acids (TFA) in dietary fats have been implicated by some researchers in the etiology of various metabolic disorders (Hanis et al, 1989, Mensink, 1990). Therefore, the accurate estimation of TFA in edible fat products is of major concern.…”

“…PARTIAL hydrogenation of vegetable oils results inevitably in the formation of octadecenoic acid isomers with different positional and geometrical configuration of the double bond. Trans unsaturated fatty acids (TFA) in dietary fats have been implicated by some researchers in the etiology of various metabolic disorders (Hanis et al, 1989, Mensink, 1990). Therefore, the accurate estimation of TFA in edible fat products is of major concern.…”