Separation of elastin cross-links as phenylisothiocyanate derivatives

Hanis, T.; Deyl, Z.; Struzinsky, R.; Mikšı́k, Ivan

doi:10.1016/s0021-9673(01)88477-6

Cited by 9 publications

(3 citation statements)

References 15 publications

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“…To this aim, human aorta [51], human yellow ligament [52] and bovine Ligamentum nuchae or rat aorta have been often used as sources of DESs: desmopyridine and isodesmopyridine. These studies were designed to understand the correlation of the elastic properties of this tissue with age [53] and to explore possible defects in elastin metabolism [54][55][56]. To find a biochemical explanation for the dilatation of vein wall, DESs and 4-l-Hydroxyproline have been quantified by HPLC in specimens of normal and varicose, dilated and non-dilated veins [57].…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

The “History” of Desmosines: Forty Years of Debate on the Hypothesis That These Two Unnatural Amino Acids May Be Potential Biomarkers of Chronic Obstructive Pulmonary Disease

Viglio¹,

Venere²,

Cagnone³

et al. 2017

Amino Acid - New Insights and Roles in Plant and Animal

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Desmosine and isodesmosine (collectively known as desmosines), two unnatural amino acids unique to mature elastin in humans, have been widely discussed as being potential biomarkers of disorders, which involve connective tissue and whose clinical manifestations result in elastin degradation. In particular, experimental data accumulated over the last 40 years have demonstrated that patients with chronic obstructive pulmonary disease (COPD) excrete higher amounts of urinary desmosines than healthy controls. Based on this evidence, it has been speculated by several authors that these cross-links may be potential biomarkers of COPD with clinical significance. Nevertheless, a strict correlation between the amount of these amino acids and the severity of the disease still has to be demonstrated. For this reason, the debate on the opportunity to consider desmosines as biomarkers of COPD is still open, and the development of sophisticated methods aimed at obtaining very precise measurement of their concentration is still considered technically challenging. The aim of this chapter is to trace the history of this debate through the presentation and discussion of a large number of articles dealing with the detection and quantification of desmosines in different biological fluids, from early years until the present.

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Section: High-performance Liquid Chromatographymentioning

confidence: 99%

The “History” of Desmosines: Forty Years of Debate on the Hypothesis That These Two Unnatural Amino Acids May Be Potential Biomarkers of Chronic Obstructive Pulmonary Disease

Viglio¹,

Venere²,

Cagnone³

et al. 2017

Amino Acid - New Insights and Roles in Plant and Animal

View full text Add to dashboard Cite

show abstract

“…Human elastin, rat aorta, and bovine ligamentum nuchae were the sources of desmosines chosen by Hanis et al [78] to develop a new method for their quantitation in tissues. Collagen was removed by treating tissues with 10% cold TCA and samples were submitted to hydrolysis in 6 N HCl.…”

Section: Hplcmentioning

confidence: 99%

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Viglio

Annovazzi

Luisetti

et al. 2007

J of Separation Science

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Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers advances in immunochemical, chromatographic, and electrophoretic procedures applied in the last 25 years to detect and quantitate these crosslinksin a variety of biological samples. Recent applications of CE with LIF detection (CE-LIF) for investigating the content of desmosines in different fluids will also be discussed.

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“…To increase the sensitivity of detection, desmosines are derivatized with trinitrophenyl [20], napthalenedialdehyde/cyanide [21], dansylchloride [22], or phenylisothiocyanate [23, 24]. …”

Section: Introductionmentioning

confidence: 99%

Cation exchange HPLC analysis of desmosines in elastin hydrolysates

et al. 2011

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Desmosine crosslinks are responsible for the elastic properties of connective tissues in lungs and cardiovascular system and are often compromised in disease states. We developed a new, fast, and simple cation exchange HPLC assay for the analysis of desmosine and isodesmosine in animal elastin. The method was validated by determining linearity, accuracy, precision, and desmosines stability and was applied to measure levels of desmosines in porcine and murine organs. The detection and quantification limits were 2 and 4 pmol, respectively. The run-time was 8 min. Our cation exchange column does not separate desmosine and isodesmosine, but their level can be quantified from absorbance at different wavelengths. Using this assay, we found that desmosines levels were significantly lower in elastin isolated from various organs of immunodeficient severe combined immunodeficiency mice compared with wild-type animals. We also found that desmosines levels were lower in lung elastin isolated from hyperhomocysteinemic Pcft −/− mice deficient in intestinal folate transport compared with wild-type Pcft +/+ animals.Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007/s00216-011-5346-z and is accessible for authorized users.

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Separation of elastin cross-links as phenylisothiocyanate derivatives

Cited by 9 publications

References 15 publications

The “History” of Desmosines: Forty Years of Debate on the Hypothesis That These Two Unnatural Amino Acids May Be Potential Biomarkers of Chronic Obstructive Pulmonary Disease

The “History” of Desmosines: Forty Years of Debate on the Hypothesis That These Two Unnatural Amino Acids May Be Potential Biomarkers of Chronic Obstructive Pulmonary Disease

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Cation exchange HPLC analysis of desmosines in elastin hydrolysates

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