Although commercial sugarcane (Saccharum spp. hybrid) produces large biomass yields, its lack of cold tolerance limits its cultivation to the tropics and subtropics. In contrast, sugarcane's close relative, Miscanthus, tolerates low temperatures. We studied 18 miscane genotypes, derived from hybridizations between two genotypes of sugarcane and two genotypes of Miscanthus (one each of M. sinensis and M. sacchariflorus). In an initial greenhouse experiment on long‐duration chilling stress (12–13°C day/7–9°C night), photosynthetic rates of the Miscanthus parents were significantly higher than the sugarcane parents after 7 days of chilling and were more than double by 14 days. The Miscanthus also retained more of their prechilling (22–25°C day/13–15°C night) photosynthetic rates (68%–72% 7 days, 64%–66% 14 days) than the sugarcanes (27% 7 days, 19%–20% 14 days). Seven of 18 miscanes exhibited higher photosynthetic rates than their sugarcane parents after 7 days of chilling, whereas after 14 days only four miscane genotypes had significantly higher photosynthetic rates than their sugarcane parents, but notably two of these did not differ from their highly tolerant Miscanthus parents. In a subsequent growth chamber experiment to evaluate short‐duration chilling stress and postchilling recovery, three miscanes representing the range of responses observed in the greenhouse experiment were compared with their parents. After 4 days of chilling (12/7°C day/night), the miscanes retained between 45% and 60% of their prechilling photosynthetic rate, with the best entry not significantly different from its Miscanthus parent (66%), and all three miscanes performed significantly better than the sugarcane parents (32%–33% for sugarcanes). After 7 days of postchilling recovery (26/18°C day/night), the Miscanthus parents and two of the miscanes fully recovered their prechilling photosynthetic rates but the sugarcane parents only recovered 69%–73% of their prechilling rates. Thus, genes from Miscanthus can be used to improve chilling tolerance of sugarcane via introgression.

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The spotted lanternfly, a newly invasive insect in the U.S. that is a great concern for the grapevine industry, produces damage on its host plants through aggressive feeding, using a piercing and sucking method to feed on the phloem of plants. In the eastern US, adult SLF can invade vineyards through fruit ripening until the end of the growing season; however, it is still unclear how prolonged late-season SLF feeding can affect the health of grapevines, as well as the host responses to this extensive damage. Thus, we have performed a comprehensive genome-wide transcriptome analysis in grapevines heavily infested by the spotted lanternfly, as it occurs in Pennsylvania vineyards, and compared it to other relevant transcriptomes in grapes with different degrees to susceptibility to similar pests. Among a variety of plant responses, we highlight here a subset of relevant biological pathways that distinguish or are common to the spotted lanternfly and other phloem feeders in grapevine. The molecular interaction between spotted lanternfly and the vine begins with activation of signal transduction cascades mediated mainly by protein kinase genes. It also induces the expression of transcription factors in the nucleus, of other signaling molecules like phytohormones and secondary metabolites, and their downstream target genes responsible for defense and physiological functions, such as detoxification and photosynthesis. Grapevine responses furthermore include the activation of genes for cell wall strengthening via biosynthesis of major structural components. With this study, we hope to provide the regulatory network to explain effects that the invasive spotted lanternfly has on grapevine health with the goal to improve its susceptibility.