Carbon dots doped with sulfur and nitrogen (S,N-CDs) were utilised to design a paper-stripe based fluorescent probe for the detection of bilirubin. The S,N-CDs were synthesized through a microwave assisted route by using citric acid as carbon source and L-cysteine as a source of nitrogen and sulfur. The S,N-CDs exhibit bright blue fluorescence emission with a peak at 452 nm. Fluorescence is quenched by Fe(III) but selectively restored by bilirubin. The quenched fluorescent probe exhibit significant selectivity and sensitivity for bilirubin in the 0.2 nM to 2 nM concentration range, with a 0.12 nM detection limit. The method was applied to the determination of bilirubin in spiked human serum and urine samples. The method was used to design a paper based test stripe as a point of care device for visual bilirubin detection. Graphical abstract Schematic representation of sulphur and nitrogen doped carbon dots whose fluorescence is quenched by Fe(III) and turned on by bilirubin. Photograph of the corresponding system under day light and UV shows the feasibility of the phenomenon. The applicability of the assay was further extended by impregnating the probe on a filter paper.

Steady-state and time-resolved fluorescence anisotropy measurements using probes coumarin 153 (C153) and 4-heptadecylumbelliferon (HUF) have been carried out to understand the micelle to gel transition of an aqueous triblock copolymer P123 ((EO)(20)-(PO)(70)-(EO)(20)) (EO = ethylene oxide; PO = propylene oxide) solution. Anisotropy results with a normal fluorescent probe, C153, do not show a characteristic change due to the micelle to gel transition. However, the probe HUF having a long hydrocarbon chain that helps its strong association with the micelle shows an increase in anisotropy above the sol-gel transition point. This difference has been explained as invoking a substantial contribution from the micellar structural fluctuations to the depolarization of HUF as its hydrocarbon chain is embedded in the micellar structure, which is not sensed significantly by the normal probe C153. That the extent of change in anisotropy for HUF upon gelation is not that large is possibly caused by the collective motion of the physically interconnected nodes, as observed from the dynamic light scattering studies, which acts in favor of a relatively faster depolarization in the gel phase. Similar studies in other copolymers, such as P85 ((EO)(26)-(PO)(40)-(EO)(26)) and F127 ((EO)(100)-(PO)(65)-(EO)(100)), further demonstrate the potential of probes latched with hydrocarbon chains in displaying a characteristic change for the micelle to gel transition which otherwise remains obscured for normal fluorescent probes.

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For a suspected forgery that involves the falsification of a document or its contents, the investigator will primarily analyze the document’s paper and ink in order to establish the authenticity of the subject under investigation. As a non-destructive and contactless technique, Hyperspectral Imaging (HSI) is gaining popularity in the field of forensic document analysis. HSI returns more information compared to conventional three channel imaging systems due to the vast number of narrowband images recorded across the electromagnetic spectrum. As a result, HSI can provide better classification results. In this publication, we present results of an approach known as the t-Distributed Stochastic Neighbor Embedding (t-SNE) algorithm, which we have applied to HSI paper data analysis. Even though t-SNE has been widely accepted as a method for dimensionality reduction and visualization of high dimensional data, its usefulness has not yet been evaluated for the classification of paper data. In this research, we present a hyperspectral dataset of paper samples, and evaluate the clustering quality of the proposed method both visually and quantitatively. The t-SNE algorithm shows exceptional discrimination power when compared to traditional PCA with k-means clustering, in both visual and quantitative evaluations.

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