S,N-doped carbon dots as a fluorescent probe for bilirubin

Abstract

Carbon dots doped with sulfur and nitrogen (S,N-CDs) were utilised to design a paper-stripe based fluorescent probe for the detection of bilirubin. The S,N-CDs were synthesized through a microwave assisted route by using citric acid as carbon source and L-cysteine as a source of nitrogen and sulfur. The S,N-CDs exhibit bright blue fluorescence emission with a peak at 452 nm. Fluorescence is quenched by Fe(III) but selectively restored by bilirubin. The quenched fluorescent probe exhibit significant selectivity and sensitivity for bilirubin in the 0.2 nM to 2 nM concentration range, with a 0.12 nM detection limit. The method was applied to the determination of bilirubin in spiked human serum and urine samples. The method was used to design a paper based test stripe as a point of care device for visual bilirubin detection. Graphical abstract Schematic representation of sulphur and nitrogen doped carbon dots whose fluorescence is quenched by Fe(III) and turned on by bilirubin. Photograph of the corresponding system under day light and UV shows the feasibility of the phenomenon. The applicability of the assay was further extended by impregnating the probe on a filter paper.

Sensitive detection of free bilirubin in blood serum using β-diketone modified europium-doped yttrium oxide nanosheets as a luminescent sensor

“…The bioelectrode was successfully applied to measure the bilirubin content in spiked serum samples. Among the methods listed in Table 1, only the S,Ndoped carbon dots-based method 14 and the method developed in this study can be used to prepare a solid-state sensor. The sensor made from S,N-doped carbon dots has a very high sensitivity; however, its detection range is not suitable to detect BR IX in humans.…”

“…14 In most of these methods, the sample needs to be pretreated; moreover, the process of detection is complicated and indirect. The accuracy of detection using a bio-enzymatic method depends on a series of environmental conditions such as pH and temperature, in addition to the inconvenient processes of extraction and storage of bio-enzymes.…”

Polyethylene Glycol6000/carbon Nanodots as Fluorescent Bioimaging Agents

“…Moreover, based on their redox potential, they can distinguish cancer cells from normal cells [29,30]. Available literature collectively and strongly suggests quantum dots to be potent as sensitive, efficient and cost-effective diagnostic or imaging nanoprobes [31,32].…”

Fluorescence enhancement of carbon dots by graphene for highly sensitive detection of tetracycline hydrochloride

“…[23][24][25] SAMs can be employed to easily control the distance between graphene and uorophores with a polyelectrolyte. instance, dopamine, bilirubin, and L-/D-arginine) [31][32][33] and proteins. 34 In this study, we synthesized poly(diallyldimethylammonium) chloride functionalized graphene (PDDA-G) with different reduction degrees to study its uorescence enhancement efficiency.…”