Sensitive detection of free bilirubin in blood serum using β-diketone modified europium-doped yttrium oxide nanosheets as a luminescent sensor

Abstract

Free bilirubin, when present in excess in the human body, can cause a multitude of diseases and disorders and even be fatal; hence, detecting it is of paramount importance. Herein, we report a luminescence quenching-based non-enzymatic method for the convenient, reliable, and rapid detection of free bilirubin in blood serum samples using sensing films (nanosheets/PS, nanosheets-tta/PS, and nanosheets-dbt/PS) as luminescent sensors. The luminescence intensity of the sensing films is linearly related to the free bilirubin concentration. Nanosheets-tta/PS demonstrated excellent sensing properties for the sensitive and reliable detection of free bilirubin in the range of 0.0-60.0 mM with a correlation coefficient of 0.9915, as compared to nanosheets/PS or nanosheets-dbt/PS. The limit of detection for the determination of free bilirubin was 41 nM. This method can be used to design a sensor-based test spot as a medical detection device for the visual detection of free bilirubin.

Tb(iii)-doped nanosheets as a fluorescent probe for the detection of dipicolinic acid

“…Furthermore, such nanosheets can be used to prepare highly responsive uorescent sensors. As reported previously, sensors based on Eu(III) or Tb(III)-doped nanosheets can sensitively and reliably detect bilirubin in human serum samples 26,27 and in dissolved or gaseous oxygen. 28 These advantageous properties motivated us to develop a novel uorescence probe based on nanosheets doped with 1% Tb(III) ions, as reported in this paper, to be used for the detection of Bacillus anthracis with high sensitivity and selectivity.…”

Efficient detection of a biomarker for infant jaundice by a europium(iii)-organic framework luminescence sensor

“…Linear range/mM LOD/nM K sv /M À1 BAMD 3 1 Â 10 À6 -500 0.0028 3.3 Â 10 10 HSA-AuNCs 7 1-50 248 AE 12 5.5 Â 10 5 Nanosheets-tta/PS 13 0-60 41 -S,N-CDs/Fe(III) 15 0.2 Â 10 À3 -2 Â 10 À3 0.12 -BSA-CuNCs/Fe 3+ (ref. 16 a direct PET from 1 to bilirubin can easily occur, responsible for the decay of the 1 at 616 nm.…”

“…Nevertheless, the Ln-MOFderived sensors for bilirubin response have been scarcely reported. [13][14][15][16]30 Herein, to gain a clinical applicable sensor for bilirubin recognization, a crystalline europium(III)-based MOF, {[Eu(H 2 O)(HCOO)(bpydb)]$solvent} n (1), was solvothermally generated by incorporation of a bulky p-conjugated 4,4 0 -(4,4 0 -bipyridine-2,6-diyl)dibenzoic acid (H 2 bpydb) as light-responsive ligand. As expected, possessing excellent thermal and environmental stability, 1 can emit bright red luminescent under the irradiation of the low-energy visible light (l ex ¼ 432 nm), and can quickly and repeatedly distinguish trace amount of bilirubin through luminescent decay with strong quenching constant (K sv ¼ 6.40 Â 10 4 M À1 ) and low detection limit (1.75 mM).…”

“…To date, a variety of available methods including diazo, 9 enzymatic, 10 vanadate oxidation, 11 electrochemical 12 and fluorometric techniques 13 have been consequently applied to recognize bilirubin in human serum and urine samples. Most of these approaches are time-consuming, involve complicated pre-treatment and depend on expensive instruments.…”

Lysozyme-Decorated Gold and Molybdenum Bimetallic Nanoclusters for the Selective Detection of Bilirubin as a Jaundice Biomarker

“…Graphene–Au NCs were successfully integrated with a surface-enhanced Raman spectroscopic technique for the detection of BR . A luminescence sensing strategy was developed for the identification of BR in blood samples using β-diketone-functionalized Eu 3+ -ion-doped Y 2 O 3 nanosheets as a probe . BSA and human serum albumin (HSA) were used as templates for the preparation of quantum dots and Cu NCs for the identification of BR in biofluids. , Even though some of the methods showed high sensitivity for the assay of BR, they required specific materials/precursors for the fluorescence assay of BR.…”