Background and Objectives:Variety of pathological factors including viral hepatitis, alcohol and drug abuse, metabolic diseases, autoimmune diseases and congenital abnormalities can cause hepatic injury. Liver transplantation is the treatment of choice for end-stage liver diseases, however, it faces several difficulties. So the aim of the work is to evaluate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the liver structure in carbon tetra chloride CCL4 induced liver fibrosis in rats.Materials and Results:BM-MSCs were isolated and characterized from long bones of twenty male albino rats. Sixty female rats were divided into the following two groups: Group I; thirty rats which were the control group. Group II; thirty rats were injected intra-peritoneal (IP) by CCL4 twice weekly for four weeks and was further subdivided into the following three subgroups: Subgroup IIA (CCL4 alone); included ten rats which were sacrificed after this four weeks. Subgroup IIB (CCL4/MSCs); included ten rats which were IP injected by a single dose of BM-MSCs and were sacrificed after four weeks. Subgroup IIC (CCL4/recovery); included ten rats which were left for another four weeks without any intervention. Histological examination of liver specimens showed that CCl4 caused variable pathological changes with elevated liver enzymes. Injection of BM-MSCs revealed an improvement in the histological picture of the liver and its enzymatic profile. On the other hand, most of the pathological lesion were still detected in rats of recovery group.Conclusions:BM-MSC could restore the liver structure and function in experimental model of liver fibrosis.
which wounded diabetic rats were treated with bone marrow-derived mesenchymal stem cells. Diabetes was induced in rats by means of a single intraperitoneal injection of streptozotocin. Wounds of groups III and IV were examined after 3 and 7 days. Skin specimens were processed and stained with H&E and Masson's trichrome. ResultsRe-epithelization with complete closure of the wound was noted in the stem celltreated group after 7 days. There was a significant increase in the number of hair follicles/high-power field and increase in the collagen content of the dermis in the stem cell-treated groups compared with the untreated groups. ConclusionBone marrow-derived mesenchymal stem cells can be effectively used in the treatment of diabetic wounds.
Introduction:The term photoaging describes the sun damaging effects on skin mainly due to chronic ultraviolet (UV) light exposure. The unsatisfactory results of the available anti-aging strategies raise the demand for alternative forms of treatments. Adipose-derived stem cells (ASCs) are available in abundant quantities, harvested by a minimally invasive procedure, safely transplanted and differentiated along multiple cell lineages. Subsequently, used in treatment of many diseases. Aim: To assess the potential ability of ASCs to ameliorate skin changes induced by chronic exposure to artificial light source similar to the sun rays in its UVA and UVB spectrum in adult female guinea pigs. Material and Methods: Adipose-derived stem cells were isolated from subcutaneous white adipose tissue of five adult human donors undergoing elective liposuction surgery. Twenty adult female guinea pigs were used and were randomly divided into two groups each was subdivided into two subgroups "five animals, each". Subgroup IA served as the control group. Subgroup IB was intradermally injected with phosphate buffered saline solution. Subgroup IIA served as the photoaging model. Subgroup IIB served as the photoaging model intradermaly injected with ASCs. Isolated stem cells were cultured and characterized. Skin specimens were prepared and examined using different histological and immunohistochemical techniques. Morphometric and statistical studies were also performed. Results: Subgroup IIA showed various UV damaging effects in the skin epidermis and dermis, while ASCs injection in subgroup IIB resulted in partial restoration of the skin structure. Conclusion: Intradermal injection of ASCs partially ameliorated the photo-damaging effects. Further studies are needed before ASCs clinical application.
Mesenchymal stem cells (MSCs) are described as multipotent cells because of their ability to differentiate into a variety of different cells and tissue lineages. AimThe aim of this study was to characterize bone marrow-mesenchymal stem cells (BM-MSCs) from male albino rats and examine their ability to differentiate into osteogenic and chondrogenic lineages. Materials and methodsBone marrow cells were cultured using complete medium. When the primary culture became nearly confluent, the adherent cells were prepared for Giemsa staining, immunostaining for CD44, CD29, and CD34, and transmission electron microscopy examination. To examine the differentiation potential of BM-MSCs, two cell pellets were prepared. One pellet was incubated in osteogenic-conditioned medium and the other pellet was incubated in chondrogenic-conditioned medium. ResultsNine days from primary culture, the adherent cells were seen as a dense homogenous population of fibroblast-like cells. The immunostaining of MSCs revealed a positive reaction for CD44 and CD29 and a negative reaction for CD34. Ultrastructural examination revealed that the MSCs had many pseudopodia and eccentric and euchromatic nuclei. Their cytoplasm was rich in free ribosomes, mitochondria, and rough endoplasmic reticulum. Examination of BM-MSCs in osteogenicconditioned medium showed calcium deposition with Alizarin red staining, whereas in chondrogenic medium these cells showed positive immunoreaction for type II collagen and bluish cytoplasmic reaction for chondroitin sulfate with Alcian blue staining. ConclusionMSCs were characterized by their morphology and CD44 and CD29 surface markers. They also had the capacity to differentiate into osteogenic and chondrogenic lineages.
Introduction Achilles tendon tears cause severe impairment in patient mobility and productivity, causing significant reduction in the quality of life. Many complications are associated with the tendon healing process such as peritendinous adhesions and excessive fibrotic scars. Unsatisfactory results appeared with the existing medical and surgical treatments to regain full tendon structure and function. Amniotic membrane is avascular, and characterized by low immunogenicity, anti-inflammatory, antiscarring properties. These criteria render it as a natural biological substitute and a novel therapeutic alternative for tendon tears. Aim: The aim of the work was to study the effect of human amniotic membrane graft application on the repair of induced Achilles tendon tear. Material and methods Fresh human amniotic membrane (AM) grafts were prepared from harvested human full-term caesarian sections-delivered placentas. Thirty adult male albino rats were divided into 3 equal groups (n = 10); group I (control group), group II (tendon tear group) and group III (AM treated group). After anesthesia, a full thickness transverse incision was induced in the rat right Achilles tendons of group II and III. Human derived amniotic membrane graft measuring 1 cm2 was applied circumferentially on the tendon tear in group III. Rats were sacrificed after 28 days. Results After the tendon tear, the untreated group (II) showed gradual accumulation of fat cells replacing the collagen bundles in focal areas. Areas of mononuclear cellular infiltration were demonstrated. The AM-treated group showed many thick parallel regularly arranged collagen fibers with a significant increase in the collagen fibers area percentage. It also showed apparent increase in tenoblasts with regular organization and apparent decrease of mononuclear inflammatory cells. Conclusion This study demonstrated the potential therapeutic role of the application of human amniotic membrane grafts in the repair of Achilles tendon tears, suggesting a future alternative therapy for patients suffering from Achilles tendon tears.
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