Background and Objectives:Variety of pathological factors including viral hepatitis, alcohol and drug abuse, metabolic diseases, autoimmune diseases and congenital abnormalities can cause hepatic injury. Liver transplantation is the treatment of choice for end-stage liver diseases, however, it faces several difficulties. So the aim of the work is to evaluate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the liver structure in carbon tetra chloride CCL4 induced liver fibrosis in rats.Materials and Results:BM-MSCs were isolated and characterized from long bones of twenty male albino rats. Sixty female rats were divided into the following two groups: Group I; thirty rats which were the control group. Group II; thirty rats were injected intra-peritoneal (IP) by CCL4 twice weekly for four weeks and was further subdivided into the following three subgroups: Subgroup IIA (CCL4 alone); included ten rats which were sacrificed after this four weeks. Subgroup IIB (CCL4/MSCs); included ten rats which were IP injected by a single dose of BM-MSCs and were sacrificed after four weeks. Subgroup IIC (CCL4/recovery); included ten rats which were left for another four weeks without any intervention. Histological examination of liver specimens showed that CCl4 caused variable pathological changes with elevated liver enzymes. Injection of BM-MSCs revealed an improvement in the histological picture of the liver and its enzymatic profile. On the other hand, most of the pathological lesion were still detected in rats of recovery group.Conclusions:BM-MSC could restore the liver structure and function in experimental model of liver fibrosis.
Recent reports have suggested that diabetic complications have a common etiology involving oxidative stress [7]. The Egyptian Journal of Histology 2012, 35:812-821 73 (1392-2012) ConclusionGarlic could protect against diabetes-induced structural changes in the renal cortex.
Background: Fructose consumption has largely increased over the past few decades. Recent studies suggested that overconsumption of fructose might increase the risk of obesity, metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). Aim of the work was to evaluate the effect of high fructose diet (HFD) on the liver structure and the possible protective role of cinnamon. Material &methods: Twenty adult male albino rats were used and equally divided into four groups. Group I; control rats were fed control diet, groupII; rats were fed control diet in concomitant with oral administration of cinnamon extract (80mg/kg/day), group III; rats were fed HFD and group IV; rats were fed HFD in concomitant with oral administration of cinnamon extract (80mg/kg/day). All rats were sacrificed after 60 days and the liver was processed for light and electron microscopic examination. Results: The liver of rats of group III (HFD group) showed cytoplasmic vacuolation of the hepatocytes around the central vein with a significant increase in the area percentage of the collagen fibers in between the hepatocytes as compared to that of the control group. Electron microscopy examination of the liver of rats of group III (HFD group) revealed numerous swollen mitochondria with loss of their cristae. Collagen fibrils in close proximity to hepatic stellate cell (HSC) and macrophage were frequently seen in between the hepatocytes. The liver of rats of group IV (HFD and cinnamon) revealed structural profile comparable to that of the control group. Conclusion: the present work revealed that HFD produced a remarkable injurious effect on the liver structure which was markedly ameliorated by concomitant cinnamon extract administration. Reduction of the daily consumption of HFD and increase of cinnamon intake is highly recommended
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.