The effect of bone marrow-derived mesenchymal stem cells on the healing of experimental skin wound in diabetic adult male albino rats

Abstract

which wounded diabetic rats were treated with bone marrow-derived mesenchymal stem cells. Diabetes was induced in rats by means of a single intraperitoneal injection of streptozotocin. Wounds of groups III and IV were examined after 3 and 7 days. Skin specimens were processed and stained with H&E and Masson's trichrome. ResultsRe-epithelization with complete closure of the wound was noted in the stem celltreated group after 7 days. There was a significant increase in the number of hair follicles/high-power field and increase in the collagen content of the dermis in the stem cell-treated groups compared with the untreated groups. ConclusionBone marrow-derived mesenchymal stem cells can be effectively used in the treatment of diabetic wounds.

Comparison between the Effects of Stem Cells and Lepidium Sativum on the Healing of Extraction Socket in Albino Rats (Histological and Immunohistochemical Study)

“…(4) The prominent feature in the stem cell group was the presence of new blood vessels with well-defined endothelium. This finding coincides the study conducted by Mohammed et al (24) who reported that BM-MSCs promote healing in rats, with increased angiogenesis because of the raised level of growth factors and the endogenous signals that accelerate the healing. Moreover, BM-MSCs are capable of generating a huge number of endothelial cells both in vitro and in vivo.…”

The role of hesperidin on healing an incised wound in an experimentally induced diabetic adult male albino rats. Histological and Immunohistochemical Study

“…The underlying dermis revealed disorganized collagen fibers and increased spaces between collagen fibers with invasion with inflammatory cells, these were in accordance with pervious research [24] . In confirmation to these results, the abnormalities in the formation of collagen protein at the wound area made healing process delayed and abnormal [20] .…”

“…These findings were explained by previous study which stated that disturbances in the functions of inflammatory cells (migration and phagocytosis), release of lytic proteases, increased production of reactive oxygen species and apoptosis. Also, diminished entrance of new blood vessels into the wound area leaded to further limitation of inflammatory cells functions [24] .…”

“…These findings were in harmony with a former research [25] that detected hypertrophic dermis with a greater degree of fibrosis in the diabetic ulcer. It could be explained as a compensatory attempt to overcome the skin impairment by initial deposition of disorganized collagen filling the wound gap [24] . These results were in harmony with E.M. findings in group III and confirmed by former study [20] .…”

“…Also, epidermal cells appeared resting on a discontinuous basement membrane and it could be explained by apoptosis [26] . In addition, these degenerative changes in keratinocytes could be explained by ischemia due to diabetes [24] . Group III showed an abundance of dilated cisternae of rough endoplasmic reticulum of fibroblasts.…”