Aim: This study is to evaluate in vitro the efficiency of Triclosan and Aloe Vera dentifrices in reducing the contamination of toothbrush bristles by a standard strain of E.coli after brushing. Settings and Design: Methods and Material: Nine Toothbrushes were divided, randomly, into 3 brushing groups, with 3 toothbrushes in each group (i.e) no dentifrice, dentifrice containing Aloe vera (Danthkanthi(R)) and dentifrice containing Triclosan (Colgate total(R)) An initial culture was made with the standard strain of E.coli(ATGC 25922) in a nutrient broth which was incubated. The dentifrices were distributed over the bristles with the tip cut from its handle and brushing was performed for 1 min on autoclaved teeth. The trimmed bristles of each toothbrush were stored in individual test tubes which were kept incubated at 37°C for 48 hrs. Streaking was done in Nutrient Agar medium kept incubated at 37°C for 24 h. The number of colony forming units was manually counted. Results: The number colony forming units is highest in the control group and the least in Triclosan containing dentifrice. Conclusions: It can be concluded that both Aloe vera and Triclosan can be used as dentifrices that help reduce E.coli count on toothbrushes.
Variability, heritability and genetic advance were studied in eight varieties of toria (Brassica compestris var. toria). The genotypic coefficient of variation (GCV) was highest for 1000 seed weight and high for number of primary and secondary branches per plant. The highest phenotypic coefficient of Variation (PCV) was observed for number of secondary branches followed by number of capsules per plant, number of primary branches per plant, 1000 seed weight and number of seeds per capsule. The highest heritability was observed for 1000 seed weight followed by plant height, number of primary branches per plant, days to maturity and number of seeds per capsule. The genetic advance as % of mean was observed highest for 1000 seed weight and high for plant height and number of primary branches.
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