The bacterial flora of non-carbonated, natural mineral water from the springs to reservoir and glass and plastic bottles

Bischofberger, Th.; S.K, SINGH; Schmitt, R. E.; König, Bernhard; Schmidt‐Lorenz, W.

doi:10.1016/0168-1605(90)90039-8

Cited by 73 publications

(66 citation statements)

References 21 publications

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“…In 1960, Buttiaux and Boudier (7) were the first to show that within 1 week after bottling and storage at ambient temperatures the natural microbial flora of the water starts to multiply and gives rise to an increase in CFU up to 10 4 to 10 5 ml Ϫ1 . Various research groups confirmed this bacterial growth phenomenon by quantifying the bacteria present in natural mineral waters at the source and at several points in time after bottling and storage at different temperatures (6,13,19,37,40,41,53). These studies focused on quantification of the bacterial community as a whole by determining (i) heterotrophic plate counts, (ii) total cell counts using acridine orange or ethidium bromide, and/or (iii) viable cell counts using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride to quantify the number of actively respiring cells.…”

mentioning

confidence: 94%

Diversity of Bacteria Growing in Natural Mineral Water after Bottling

Loy

Beisker²,

Meier

2005

Appl Environ Microbiol

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Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bottling. Seven 16S rRNA gene libraries, comprising 108 clones in total, were constructed from water samples taken at various days after bottling and from two different bottle sizes. Sequence analyses identified 11 operational taxonomic units (OTUs), all but one affiliated with the betaproteobacterial order Burkholderiales (6 OTUs) or the class Alphaproteobacteria (4 OTUs). Fluorescence in situ hybridization (FISH) was applied in combination with DAPI (4,6-diamidino-2-phenylindole) staining, viability staining, and microscopic counting to quantitatively monitor changes in bacterial community composition. A growth curve similar to that of a bacterium grown in a batch culture was recorded. In contrast to the current perception that Gammaproteobacteria are the most important bacterial components of natural mineral water in bottles, Betaproteobacteria dominated the growing bacterial community and accounted for 80 to 98% of all bacteria detected by FISH in the late-exponential and stationary-growth phases. Using previously published and newly designed genusspecific probes, members of the betaproteobacterial genera Hydrogenophaga, Aquabacterium, and Polaromonas were found to constitute a significant proportion of the bacterial flora (21 to 86% of all bacteria detected by FISH). For the first time, key genera responsible for bacterial growth in a natural mineral water were identified by applying molecular cultivation-independent techniques.

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mentioning

confidence: 94%

Diversity of Bacteria Growing in Natural Mineral Water after Bottling

Loy

Beisker²,

Meier

2005

Appl Environ Microbiol

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“…However, small DOC increases were also recorded for the filtered Agricultural and Estuary waters. Past studies have suggested that a "bottle effect" may occur with natural water samples stored in plastic or glassware, where contact with such media can artificially increase microbial numbers in water samples and lead to experimental artefacts (Bischofberger et al 1990). On the other hand, Hammes et al (2010) found no evidence of significant bottle effects across a range of bottle sizes and surface area to volume ratios.…”

Section: Susceptibility Of Contrasting Waters To Photo-degradationmentioning

confidence: 99%

Transformations in DOC along a source to sea continuum; impacts of photo-degradation, biological processes and mixing

Jones¹,

Evans

Jones³

et al. 2015

Aquat Sci

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“…However, precious little empirical data that actually quantify and explain the volumetric "bottle effect" are ever presented. In one example, Bischofberger et al (3) observed that incubation of groundwater led to significantly more growth (about 2 log units) in small bottles (100 ml) than in big ones (10 liters). More often, however, the "bottle effect" is merely mentioned, as if it is self-explanatory and indisputable (2,11,12).…”

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confidence: 99%

“…Microbial batch growth during confined incubation in bottles of various sizes is used daily in a broad variety of microbiological studies and methods, including bioassays such as the assimilable organic carbon (AOC) assay (6,10,18) and the analysis of pure culture or microbial community growth in freshwater (3,11,19,20). In this context, "bottle effect" or "volume effect" is a term that has cropped up frequently in aquatic microbiology papers (e.g., references 12, 13, and 21) during the last 100 years to explain inexplicable phenomena and variations in results obtained from such batch growth studies.…”

mentioning

confidence: 99%