SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic parameters of the interactions between the immobilised probes and the ligands in solution. We review the current state of development of SPRi technology and its application including commercially available SPRi instruments. Attention is also given to surface chemistries for biochip functionalisation and suitable approaches to improve sensitivity.

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SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic parameters of the interactions between the immobilised probes and the ligands in solution. We review the current state of development of SPRi technology and its application. Commercially available SPRi instruments are covered. Attention is also given to surface chemistries for biochip functionalisation and suitable approaches to improve sensitivity.

A simplified colloidal lithography protocol (polyelectrolyte monolayer) for Au nanohole fabrication and autocorrelation/FFT analysis of SEM images to disclose hidden short-range periodicities.

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Dopamine oxidation and self-polymerization has recently gained a large interest arising from the versatile chemistry of this endogenous catecholamine. Particularly stimulating appear the applications of this biopolymer for surface coating, molecular imprinting, and electrochemistry, here reviewed, covering the broad fields of medicine, material science, and (bio)analytical chemistry.Nonetheless, the peculiar physicochemical properties of dopamine and its polymer, due to the reduction potential of catechol moiety, are not fully exploited. We have confidence in possibility to spread its applications through a large variety of research approaches, including the use of naturally occurring or synthetic dopamine analogues and co-polymers. Accordingly, our efforts in this direction are focused in proposing the role of this polymer for quantitative applications, evaluating analytical performances, costs, reproducibility and versatility of the developed methods also revisiting standard (bio)analytical platforms.

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