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Cited by 37 publications
(34 citation statements)
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“…This LOD is not only lower than that available by the conventional fluorescence technique by a factor of ≈200 fold, but also one order of magnitude lower than those of human genomic DNA reported in refs. To date, this LOD is the lowest ever reported in fluorescence based dsDNA diagnosis to the best of our knowledge. This higher SNR stems from plasmonic enhancement of local field strength, hence heightening efficiency of coupling between dye molecules and surface plasmons at both wavelengths of the dye transitions, i.e., λ ex and λ em at the same time.…”
Section: Resultsmentioning
confidence: 84%
See 1 more Smart Citation
“…This LOD is not only lower than that available by the conventional fluorescence technique by a factor of ≈200 fold, but also one order of magnitude lower than those of human genomic DNA reported in refs. To date, this LOD is the lowest ever reported in fluorescence based dsDNA diagnosis to the best of our knowledge. This higher SNR stems from plasmonic enhancement of local field strength, hence heightening efficiency of coupling between dye molecules and surface plasmons at both wavelengths of the dye transitions, i.e., λ ex and λ em at the same time.…”
Section: Resultsmentioning
confidence: 84%
“…In this work, we present the fluorescence enhanced by SPCE with continuous thin metal films, coping with all issues mentioned above, while providing sufficient enhancement (η E )without compromising its reproducibility via continous bimetal film engineering that increases the plasmonic resonance quality factors. We use SYBR Green I, the dye widely adopted to quantify the concentration of double stranded DNA (dsDNA) in fluorescence based molecular biology . As an analyte, we choose dsDNA for the interferon‐α (IFN‐α) promoter (gene therapy application for cancer).…”
Section: Introductionmentioning
confidence: 99%
“…where σ N is the associated noise within the result (0.24nm), h is the thickness of the layer that has the refractive index change associated with the aptamer molecule (assumed here to be of the order of the size of the thrombin molecule ~5nm) and Δ n c ∆ is the volume refractive index increment of the molecular concentration, estimated to be about 0.18 cm 3 /g (DNA or BSA [39,40]) leading to 0.2pg/mm 2 . This result again is amongst the highest from an optical sensing platforms reported in the literature [22][23][24][25]41]. Detection limits of the flow device were then examined.…”
Section: Detection Of Thrombinmentioning
confidence: 74%
“…The sandwich assay was based on the optimization of sample pretreatment (fragmentation and denaturation) coupled to a rational design of very selective and performing probes. In particular, thiolated capturing probes, immobilized on the sensor surface, hybridized the target (ABCB1 gene fragment) while a secondary probe hybridization confirmed the selectivity of the capturing [105].…”
Section: Point Mutation Detectionmentioning
confidence: 90%
“…Recently (2013), Ermini et al have developed a very sensitive (140 aM) and label-free DNA sensor for the specific detection of non-amplified ABCB1 gene fragment (extracted from human lymphocytes) on SPRi Lab + platform (Horiba Scientific) [105]. ABCB1 is a key gene in pharmacogenomics, with many SNPs implicated in a common set of problems such as altered drug levels and susceptibility to diseases (e.g., inflammatory bowel disease, Parkinson's disease, refractory seizures, and CD4 cell recovery during human immunodeficiency virus therapy) [106].…”
Section: Point Mutation Detectionmentioning
confidence: 99%