Dopamine oxidation and self-polymerization has recently gained a large interest arising from the versatile chemistry of this endogenous catecholamine. Particularly stimulating appear the applications of this biopolymer for surface coating, molecular imprinting, and electrochemistry, here reviewed, covering the broad fields of medicine, material science, and (bio)analytical chemistry.Nonetheless, the peculiar physicochemical properties of dopamine and its polymer, due to the reduction potential of catechol moiety, are not fully exploited. We have confidence in possibility to spread its applications through a large variety of research approaches, including the use of naturally occurring or synthetic dopamine analogues and co-polymers. Accordingly, our efforts in this direction are focused in proposing the role of this polymer for quantitative applications, evaluating analytical performances, costs, reproducibility and versatility of the developed methods also revisiting standard (bio)analytical platforms.