Adenosine plays a crucial role in the regulation of physiological activity in various tissues and organs. As adenosine is a possible biomarker for cancer, the determination of its level presents a demanding task for deeply monitoring progress of diseases. Through the synthesis of Fe3O4/Au/Ag nanocomposites weaved and stabilized by phytic acid and its salt, we develop a magnetically assisted surface-enhanced Raman scattering (SERS) protocol to determine trace level adenosine in urine samples from both lung cancer patients and health human. The magnetic properties of the nanocomposites enable to realize the simple separation of targeted molecules from a complex matrix and the Au/Ag nanoparticles moieties act as the SERS platform. This label-free Fe3O4/Au/Ag-nanocomposites-based SERS protocol shows a good stability, reproducibility, time efficiency (less than 20 min for one sample test), and huge sensitivity down to 1 × 10(-10) M. The protocol also has high selectivity because SERS signal of adenosine provides the molecular fingerprint information as well as an azo coupling pretreatment is performed to remove the interference of urea. Furthermore, a SERS array is designed for on-site screening adenosine in urine samples in a massive way using a portable Raman. Such a magnetically assisted SERS method as a powerful alternative can be expected as a smart and promising tool for effective assessment of healthcare.
A novel magnetically responsive and surface-enhanced Raman spectroscopy (SERS) active nanocomposite is designed and prepared by direct grafting of Au nanoparticles onto the surface of magnetic network nanostructure (MNN) with the help of a nontoxic and environmentally friendly reagent of inositol hexakisphosphate shortly named as IP6. The presence of IP6 as a stabilizer and a bridging agent could weave Fe3O4 nanoparticles (NPs) into magnetic network nanostructure, which is easily dotted with Au nanoparticles (Au NPs). It has been shown firstly that the huge Raman enhancement of Au-MNN is reached by an external magnetic collection. Au-MNN presenting the large surface and high detection sensitivity enables it to exhibit multifunctional applications involving sufficient adsorption of dissolved chemical species for enrichment, separation, as well as a Raman amplifier for the analysis of trace pesticide residues at femtomolar level by a portable Raman spectrometer. Therefore, such multifunctional nanocomposites can be developed as a smart and promising nanosystem that integrates SERS approach with an easy assay for concentration by an external magnet for the effective on-site assessments of agricultural and environmental safety.
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