Precipitation has been considered to be a critical water source for both human livelihoods and ecosystems in Central Asia. Using observational data and gridded datasets, we studied the regional and seasonal differences of precipitation climate characteristics and variations in precipitation over Central Asia. Using observational data obtained from the China Meteorological Administration, Global Historical Climatology Network (V3.02), we divided Central Asia into four subregions (North, Center, Southwest, and Southeast) based on the differences in seasonal cycles of precipitation. 'Single peaks' were detected as types of seasonal cycles over the North and the Southeast, while 'two peaks' was the type that occurred in the Southwest. For the Center, the zone of transition between the North and the Southwest, each monthly precipitation value was higher than the Southwest's and less than the North's. GPCC (R 2 of 0.89, RMSE of 64.5 mm/year) was proven to be the most suitable dataset of the four datasets (CRU, GPCC, MERRA, and TRMM) to describe precipitation in Central Asia, based on validation against observational data, and used to detect the spatial and temporal trend of precipitation in Central Asia and four subregions during . No significant trends were observed for annual precipitation in Central Asia, while precipitation in winter displayed a significant increase (0.11 mm/year). Additionally, significantly increasing trends (0.16, 0.27, 0.13, and 0.13 mm/year) were detected in spring, summer, autumn, and winter over the Southeast during 1960Southeast during -2013

Based on daily precipitation measurements from 271 meteorological stations in northern China during 1960–2011, we found that precipitation amount did not show significant trend (p = 0.25). To obtain a better understanding of regional precipitation differences, we divided northern China into two regions and five sub‐regions. A wetting tendency was identified for the northwestern of China (NW), while a drying tendency was determined for the northeastern of China (NE). Precipitation tendencies also displayed regional differences. We determined that changes in precipitation frequency dominated changes in precipitation amount for low altitude areas, while changes in precipitation intensity were the main cause of changes in precipitation amount in high altitude areas. Both precipitation frequency and intensity had an equivalent impact on changes within middle altitude regions of the NW. In contrast, changes in precipitation amount within the NE were mainly caused by precipitation frequency. The effect of precipitation intensity on precipitation amount was not significant. For all of the sub‐regions investigated, heavy precipitation contributed the most to changes in total precipitation amount. The results obtained from this study will be of value to those studying the response of northern Chinese ecosystems to climate change.

During 2012–2017, we collected throat swabs from dogs in China to characterize canine influenza virus (CIV) A(H3N2) isolates. A new antigenically and genetically distinct CIV H3N2 clade possessing mutations associated with mammalian adaptation emerged in 2016 and replaced previously circulating strains. This clade probably poses a risk for zoonotic infection.

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Since the first report of the plasmid-mediated, colistin-resistant gene, mcr-1, nine mcr genes and their subvariants have been identified. The spreading scope of mcr-1~10 varies greatly, suggesting that mcr-1~10 may have different evolutionary advantages. Depending on MCR family phylogeny, mcr-6 is highly similar to mcr-1 and -2, and mcr-7~10 are highly similar to mcr-3 and -4. We compared the expression effects of MCR-1~5 on bacteria of common physiological background. The MCR-1-expressing strain showed better growth than did MCR-2~5-expressing strains in the presence of colistin. LIVE/DEAD staining analysis revealed that MCR-3~5 expression exerted more severe fitness burdens on bacteria than did MCR-1 and -2. Bacteria expressing MCRs except MCR-2 showed enhanced virulence with increased epithelial penetration ability determined by trans-well model (p < 0.05). Enhanced virulence was also observed in the Galleria mellonella model, which may have resulted from bacterial membrane damage and different levels of lipopolysaccharide (LPS) release due to MCR expression. Collectively, MCR-1-expressing strain showed the best survival advantage of MCR-1~5-expressing strains, which may partly explain the worldwide distribution of mcr-1. Our results suggested that MCR expression may cause increased bacterial virulence, which is alarming, and further attention will be needed to focus on the control of infectious diseases caused by mcr-carrying pathogens.

Equine-origin H3N8 and avian-origin H3N2 canine influenza viruses (CIVs) prevalent in dogs are thought to pose a public health threat arising from intimate contact between dogs and humans. However, our understanding of CIV virulence is still limited. Influenza A virus PA-X is a fusion protein encoded in part by a +1 frameshifted open reading frame (X-ORF) in segment 3. The X-ORF can be translated in full-length (61-amino-acid) or truncated (41-amino-acid) form. Genetic analysis indicated that the X-ORFs of equine H3N8 and avian H3N2 influenza viruses encoded 61 amino acids but were truncated after introduction into dogs. To determine the effect of PA-X truncation on the biological characteristics of CIVs, we constructed four recombinant viruses on H3N8 and H3N2 CIV backgrounds bearing truncated or full-length PA-Xs. We observed that truncation of PA-X increased growth of both H3N8 and H3N2 CIVs in MDCK cells and suppressed expression from cotransfected plasmids in MDCK cells. Furthermore, truncation of PA-X enhanced viral pathogenicity in dogs, as shown by aggravated clinical symptoms and histopathological changes, increased viral replication in the respiratory system, and prolonged virus shedding. Additionally, CIVs with truncated PA-Xs were transmitted more efficiently in dogs. Global gene expression profiling of the lungs of infected dogs revealed that differentially expressed genes were mainly associated with inflammatory responses, which might contribute to the pathogenicity of PA-X-truncated CIVs. Our findings revealed that truncation of PA-X might be important for the adaptation of influenza viruses to dogs. IMPORTANCE Epidemics of equine-origin H3N8 and avian-origin H3N2 influenza viruses in canine populations are examples of successful cross-species transmission of influenza A viruses. Genetic analysis showed that the PA-X genes of equine H3N8 or avian H3N2 influenza viruses were full-length, with X-ORFs encoding 61 amino acids; however, those of equine-origin H3N8 or avian-origin H3N2 CIVs were truncated, suggesting that PA-X truncation occurred after transmission to dogs. In this study, we extended the PA-X genes of H3N8 and H3N2 CIVs and compared the biological characteristics of CIVs bearing different lengths of PA-X. We demonstrated that for both H3N8 and H3N2 viruses, truncation of PA-X increased virus yields in MDCK cells and enhanced viral replication, pathogenicity, and transmission in dogs. These results might reflect enhanced suppression of host gene expression and upregulation of genes related to inflammatory responses. Collectively, our data partially explain the conservation of truncated PA-X in CIVs.

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Abstract:In this work, rain totals from 1960-2014, obtained during the warm season (May to October) from 52 meteorological stations, over Xinjiang, China were classified as either light, moderate, or heavy rain in two sub-regions (northern and southern). Spatial and temporal trends for rain amounts and days for the three rain classes were determined. All light, moderate, and heavy rain amounts displayed increasing trends over the two sub-regions. Furthermore, heavy rain amounts contributed the most to changes in total rain amounts. Light rain days in northern Xinjiang significantly decreased, in contrast to increasing light rain days in southern Xinjiang and moderate and heavy rain days within two sub-regions. Results obtained from correlation and relative weights analyses implied that lower-tropospheric specific humidity was the main factor responsible for light rain day trends in Xinjiang. Increasing temperatures were not found to have a significant effect.