Canine Influenza Virus A(H3N2) Clade with Antigenic Variation, China, 2016–2017

Lyu, Yanli; Song, Shikai; Zhou, Li-Wei; Bing, Guoxia; Wang, Qian; Sun, Haoran; Chen, Mingyue; Hu, Junyi; Wang, Mingyang; Sun, Honglei; Pu, Juan; Xia, Zhaofei; Liu, Jinhua; Sun, Yipeng

doi:10.3201/eid2501.171878

Cited by 13 publications

(17 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CADY1/2019 achieved a peak titer of 9.1 lgTCID 50 /mL in MDCK cells at 48 hpi. H3N2 CIV infection has already been confirmed in a human (Lyu et al, 2019). The H3N2 CIV isolated in America had limited replication in the lungs of swine (Abente et al, 2016).…”

Section: Discussionmentioning

confidence: 89%

“…All genes of CADY1/2019 were close to those of A/canine/Nanjing/20170328-7/2017 which belonged to a novel subclade emerged in 2016. And viruses in such novel subclade were reported phylogenetically and antigenically different from previously circulating CIV strains ( Lyu et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…A novel antigenic and genetic distinct clade of H3N2 CIVs was reported in China in 2016. The novel H3N2 CIVs that acquired an adaptation mutation to infect mammals, replaced previous CIVs and posed a zoonotic potential ( Lyu et al, 2019 ). CADY1/2019 belonged to such a clade.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, few CIVs fall into human/swine lineage, such as Guangxi isolates ( Chen et al, 2015 ), which indicates that those viruses might originate from swine or human influenza viruses. CIVs have become increasingly diverse ( Song et al, 2012 ; Moon et al, 2015 ; Na et al, 2015 ; Chen et al, 2018 ; Lyu et al, 2019 ; Shen et al, 2020 ) and have zoonotic potential.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Characteristics, Antigenicity, Pathogenicity, and Zoonotic Potential of a H3N2 Canine Influenza Virus Currently Circulating in South China

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Canine influenza viruses (CIVs) could be a source of influenza viruses which infect humans because canine are important companion pets. To assess the potential risk of H3N2 CIVs currently circulating in southern China to public health, biological characteristics of A/canine/Guangdong/DY1/2019 (CADY1/2019) were detected. CADY1/2019 bound to both avian-type and human-type receptors. CADY1/2019 had a similar pH value for HA protein fusion to human viruses, but its antigenicity was obviously different from those of current human H3N2 influenza viruses (IVs) or the vaccine strains recommended in the North hemisphere. CADY1/2019 effectively replicated in the respiratory tract and was transmitted by physical contact among guinea pigs. Compared to human H3N2 IV, CADY1/2019 exhibited higher replication in MDCK, A549, 3D4/21, ST, and PK15 cells. Sequence analysis indicated that CADY1/2019 is an avian-origin virus, and belongs to the novel clade and has acquired many adaptation mutations to infect other mammals, including human. Taken together, currently circulating H3N2 CIVs have a zoonotic potential, and there is a need for strengthening surveillance and monitoring of their pathogenicity.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Characteristics, Antigenicity, Pathogenicity, and Zoonotic Potential of a H3N2 Canine Influenza Virus Currently Circulating in South China

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Many previous studies have focused on the phylogenetic history of H3N2 CIV (Zhu et al, 2015;Voorhees et al, 2017Voorhees et al, , 2018He et al, 2019). In addition, several putative or confirmed adaptive mutations that separate H3N2 CIV from avian reservoir viruses or lead to antigenic changes among circulation clades had been reported previously (Yang et al, 2013;Lin et al, 2016;He et al, 2019;Lyu et al, 2019;Wu et al, 2021). Recently, the evolutionary dynamics on population size, selection pressure, and nucleotide substitution rates on virus genes of H3N2 CIV has been estimated (Shen et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Host Adaptive Evolution of Avian-Origin H3N2 Canine Influenza Virus

et al. 2021

View full text Add to dashboard Cite

Since its first isolation in around 2007, the avian-origin H3N2 canine influenza virus (CIV) has become established and continues to circulate in dog populations. This virus serves as a useful model for deciphering the complex evolutionary process of interspecies transmission of influenza A virus (IAV) from one species to its subsequent circulation in another mammalian host. The present investigation is a comprehensive effort to identify and characterize genetic changes that accumulated in the avian-origin H3N2 CIV during its circulation in the dog. We revealed that H3N2 CIV experiences greater selection pressure with extremely high global non-synonymous to synonymous substitution ratios per codon (dN/dS ratio) for each gene compared to the avian reservoir viruses. A total of 54 amino acid substitutions were observed to have accumulated and become fixed in the H3N2 CIV population based on our comprehensive codon-based frequency diagram analysis. Of these substitutions, 11 sites also display high prevalence in H3N8 CIV, indicating that convergent evolution has occurred on different lineages of CIV. Notably, six substitutions, including HA-G146S, M1-V15I, NS1-E227K, PA-C241Y, PB2-K251R, and PB2-G590S, have been reported to play imperative roles in facilitating the transmission and spillover of IAVs across species barriers. Most of these substitutions were found to have become fixed in around 2015, which might have been a favorable factor that facilitating the spread of these CIV lineages from South Asia to North America and subsequent further circulation in these areas. We also detected 12 sites in six viral genes with evidence for positive selection by comparing the rates of non-synonymous and synonymous substitutions at each site. Besides, our study reports trends of enhanced ongoing adaptation of H3N2 CIV to their respective host cellular systems, based on the codon adaptation index analysis, which points toward increasing fitness for efficient viral replication. In addition, a reduction in the abundance of the CpG motif, as evident from an analysis of relative dinucleotide abundance, may contribute to the successful evasion of host immune recognition. The present study provides key insights into the adaptive changes that have accumulated in the avian-origin H3N2 viral genomes during its establishment and circulation into dog populations.

show abstract

Epidemiological survey and genetic evolution of H3N2 subtype influenza viruses from stray dogs in Shanghai, China

Shen

et al. 2020

Virus Genes

View full text Add to dashboard Cite

Canine Influenza Virus A(H3N2) Clade with Antigenic Variation, China, 2016–2017

Cited by 13 publications

References 14 publications

Molecular Characteristics, Antigenicity, Pathogenicity, and Zoonotic Potential of a H3N2 Canine Influenza Virus Currently Circulating in South China

Molecular Characteristics, Antigenicity, Pathogenicity, and Zoonotic Potential of a H3N2 Canine Influenza Virus Currently Circulating in South China

Host Adaptive Evolution of Avian-Origin H3N2 Canine Influenza Virus

Epidemiological survey and genetic evolution of H3N2 subtype influenza viruses from stray dogs in Shanghai, China

Contact Info

Product

Resources

About