ABSTRACT--A penaeidin cDNA sequence of tiger shrimp Penaeus monodon was obtained.In the sequence, an open reading frame that coded for a peptide composed of 74 amino acids was found. A cleavage site of secretory signal peptide was predicted between amino acids 19 and 20. The calculated molecular mass of mature penaeidin was about 6.1 kDa and the estimated pl of this peptide was 9.1. Northern blot analysis indicated that the penaeidin was mainly synthe sized in the hemocytes.Clustering analysis of penaeidin sequences from P. monodon, P. vannamei, P. setiferus, P. japonicus and P. chinensis was performed. The recombinant penaeidin was expressed using insect-baculovirus expression system. The recombinant penaeidin showed antimicrobial activity against a bacterium Aerococcus viridans, but not Vibrio alginolyticus, Vibrio harveyi or a yeast Debaryomyces hansenii. In addition, it delayed spore germination and growth of a filamentous fungus Neurospora crassa.
Abstract:Understanding the driving forces of absorption of anthropogenic CO 2 by the oceans is critical for a sustainable ocean carbon cycle. Decadal sinking particle flux data collected at 1000 m, 2000 m, and 3500 m at the South East Asia Time Series Study (SEATS) Station (18 • N, 116 • E), which was located in the northern South China Sea (nSCS), show that the fluxes undergo strong seasonal and interannual variability. Changes in the flux data are correlated with the satellite-derived chlorophyll-a concentration, indicating that the mass fluxes of the sinking particles are largely controlled by the export production at or near the SEATS station. The cooling of seawater and the strengthening of wind in winter increase the nutrient inventories in the euphotic zone, thus also increasing export production in the nSCS. This study reveals that the intrusion of low-nutrient seawater from the West Philippine Sea into the nSCS significantly reduces the productivity, and hence the flux, of sinking particles.
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