Schiff base compound 5-(benzylideneamino)-1,3,4-thiadiazole-2-thiol was prepared from condensation reaction of 2-amino-5-mercapto-1,3,4-thiadiazole with benzaldehyde. New Schiff bases react with copper (II), Ferric (III), Cobalt (II) and Zinc (II) to form four complexes. The Schiff base complexes were identification by using FTIR and UV-VIS. The antibacterial activity of complexes (Copper (II) LM 1 , Ferric (III) LM 2 , Cobalt (II) LM 3 and Zinc (II) LM 4 complexes) were studied against Kocuria kristinae, Alloiococcus otiti and Aerococcus urinae as a model of Gram positive, Pseudomonas stutzeri, Ochrobactrum anthropic and Pantoea agglomerans as a model of Gram negative to determine the activity of synthesized complexes. Identification of these uncommon bacteria that isolated from urinary tract infection confirmed by using VITEK2 compact system. Several antibiotics have been chosen to investigate the ability of these isolates to resist the conventional antibiotic. The results showed higher activity of the new compounds relative to the chosen antibiotics.

Schiff base compound 5-(benzylideneamino)-1,3,4-thiadiazole-2-thiol was prepared from condensation reaction of 2-amino-5-mercapto-1,3,4-thiadiazole with benzaldehyde. Schiff bases react with Copper(ӀӀ), Ferric(ӀӀӀ), Cobalt(ӀӀ) and Zinc(ӀӀ) to form four complexes. The Schiff base complexes were identification by using FTIR and UV-VIS. The antibacterial activity of complexes (Copper(ӀӀ), Ferric(ӀӀӀ), Cobalt(ӀӀ) and Zinc(ӀӀ)) were studied against S. aureus and S. epidermidis as a model of Gram positive, E. coli, P. mirabilis, C. freundii and P. aeruginosa as a Gram negative model to determine activity of synthesized complexes, after subjected them to some tests to confirm the identity of the pathogenic bacteria. Ten antibiotics (Ampicillin and Amoxicillin) have been chosen to investigate the ability of bacterial isolates to resistant the conventional antibiotic. Imipenem have been selected to contrast its efficiency with those of the new compounds because of its high efficiency. The results exhibited higher activity of the new compounds proportional to the chosen antibiotics.

The number of people who wear contact lenses (CLS) has been especially increased in the past decade as an alternative to glasses, but many of them do not realize the extent of the hazards involved, and these risks Keratitis associated with microbial causes, so this study included isolation and identification the bacteria in fluids save to  lenses with 42 users of contact lenses of students in inner section in the Tikrit University. The study showed the presence of different bacterial groups in the fluids keeping contact lenses which included Staph. epidermis with percentage 30.30% and Staph. Aureuswith percentage 21.21% and the bacteria Streptococcus pneumonia  precentage 18.18% and also was diagnosed with Pseudomonas aeruginosa and with 9.09%, while the intestinal bacteria Serratia odorfera where it was obtained with 6.06%. As for the sensitivity of the bacterial isolates to antibiotics, the study proved that more antibiotic effect on gram positive and negative bacteria is erythromycin followed by chloramphenicol while more antibiotic resistance by bacteria is ezithromycin, due to the erroneous and excessive use of these drugs by people.