Abstract:The aim of our study was to evaluate the effect of in vitro anticancer and cytotoxic activity of the methanolic extracts of 14 medicinal plants, 8 of which are endemic species in Anatolia, against the human HeLa cervical cancer cell line and to compare to the normal African green monkey kidney epithelial cell line (Vero) using the MTT colorimetric assay. Values for cytotoxicity measured by MTT assay were expressed as the concentration that causes 50% decrease in cell viability (IC50, μg/mL). The degree of selectivity of the compounds can be expressed by its selectivity index (SI) value. High SI value (>2) of a compound gives the selective toxicity against cancer cells (SI = IC50 normal cell/IC50 cancer cell). Dose-dependent studies revealed IC50 of 293 mg/mL and >1000 mg/mL for Cotinus coggygria Scop., IC50 of 265 μg/mL and >1000 mg/mL for Rosa damascena Miller, IC50 of 2 μg/mL and 454 mg/mL for Colchicum sanguicolle K.M. Perss, IC50 of 427 μg/mL and >1000 μg/mL for Centaurea antiochia Boiss. var. praealta (Boiss & Bal) Wagenitz on the HeLa cells and the Vero cells, respectively. Four plants showed significant SI values which were 227 for Colchicum sanguicolle K.M. Perss (endemic species), >3.8 for Rosa damascena Miller, >3.4 for Cotinus coggygria Scop. and >2.3 for Centaurea antiochia Boiss. var. praealta (Boiss & Bal) Wagenitz (endemic species). According to our study, 4 methanolic extracts of 14 tested plants exhibit greater activity on the HeLa cell line and little activity on the Vero cell line, meaning that these plants can be evaluated for potential promising anticancer activity.
The aim of our study was to evaluate in vitro antioxidant potential of methanolic extracts (ME) of 14 medicinal plants, 8 of which are endemic species of Anatolia. Scavenging activity was tested by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and the inhibitory effect on lipid peroxidation was examined by the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. The obtained results showed significant differences in the antioxidant potential amongst the tested methanolic plant extracts. Among the plant samples, Crataegus microphylla C. Koch, Salvia hypargeia Fisch. & Mey., Cotinus coggygria Scop., Origanum sipyleum L. and Rosa damascena Miller exhibited the highest DPPH scavenging activity. Five extracts (Centaurea nerimaniaeŞ. K€ ult€ ur, C. coggygria, Scorzonera tomentosa L., R. damascena and Colchicum sanguicolle K.M. Perss) showed strong antioxidant activity in the FTC and TBA tests, with per cent inhibition ranges of 72%À84% and 84%À92%, respectively. The ME of C. coggygria and R. damascena exhibited potent antioxidant activity by the DPPH, FTC and TBA methods.
Turkey has many plants used by public for the treatment of various diseases. The usage of these folk medicine which is prepared by these plants has been transferred from generation to generation. Ethnobotanical studies carried out by traditional methods of treatment are recorded and this information is aimed to contribute to the development of the drug. ın this study, it has been reached to 77 taxa that is used in traditional treatment against anemia in Turkey and aimed to give information about scientific and local names of these taxa, families, used parts and usage in anemia.
The antimicrobial and cytotoxic effects of various endemic and non-endemic Eryngium species from Turkey were investigated. Nine endemic and two non-endemic Eryngium species were analyzed using a microdilution assay for their antibacterial and antifungal activities. Cytotoxic activities of E. pseudothoriifolium, E. thorifolium, E. davisii, and E. falcatum, on prostate carcinoma and endometrial cancer cells were analyzed by cytotoxic activity assay. It was observed that 16 out of 22 extracts (aerial or root part) showed antibacterial activities. Fifteen out of 22 extracts showed antifungal activity with the lowest MIC (156 mg/l) value against Candida albicans ATCC 10231 and C. tropicalis ATCC 750. Aerial parts of E. pseudothoriifolium, E. thorifolium, E. davisii, and E. falcatum exhibited cytotoxic effects on endometrial cancer cells. This evaluation of tested endemic Eryngium species’ antimicrobial and cytotoxic activities is the first of its kind.
Turkey has a rich plant diversity due to its geography and many of these plants are used in the treatment of various diseases. The usage of these medicinal plants has been passed down from generation to generation. Ethnobotanical studies are used to record traditional treatment methods and this information is intended to contribute to drug development studies. This study (which is the fourth article in a series focusing on plants with painkilling properties) details the plants used to combat toothache. In this survey, which was prepared by screening ethnobotanical research, a total of 52 taxa were traditionally used in toothache treatment in Turkey. Scientific and local names, families, parts used, and treatment methods of these plants were recorded. According to the results of the research, the most common families are Lamiaceae, Asteraceae, Solanaceae, Euphorbiaceae, Liliaceae and Rosaceae. The most commonly used taxa in different regions of Turkey are Cornus mas, Dianthus zonatus var. zonatus and Hyocyamus niger. These plants contain analgesic and anti-inflammatory compounds. These plants are usually used externally as extracts (infusion/decoction) as well as are used directly. However, a few species are also used externally.
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