<i>In vitro</i>evaluation of antioxidant activity of some plant methanol extracts

Karagöz, Ali; Artun, Fulya Tugba; Özcan, Gül Sinemcan; Melikoğlu, Gülay; Anil, Sezin; Kültür, Şükran; Sütlüpınar, N.

doi:10.1080/13102818.2015.1080600

Cited by 38 publications

(23 citation statements)

References 20 publications

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“…With regard to previously described biological properties, multiple O. sipyleum extracts were found to display scavenging effect against DPPH and hydroxyl radicals, and were also able to reduce the formation of peroxides, in thiocyanite method, and molybdenum (VI) to molybdenum (V) in, phosphomolybdenum method (Karagöz et al, ; Nakiboglu, Urek, Kayali, & Tarhan, ; Ozkan et al, ). Moreover, flower methanol extract was effective as antimicrobial agent.…”

Section: Introductionmentioning

confidence: 91%

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

et al. 2019

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The phytochemical, antiradical, and enzyme inhibition profile of three solvent extracts (ethyl acetate, methanol, water) of Origanum sipyleum were assessed. We also performed a pharmacological study in order to explore protective effects induced by extracts in inflamed colon. LC‐MS analysis revealed that the extracts contained different classes of phenolics. The aqueous extract showed the highest antioxidant and acetylcholinesterase (AChE) inhibitory effects. Total phenol and flavonoid contents were highest in aqueous and ethyl acetate extract, respectively. All extracts were effective in reducing colon pro‐oxidant and pro‐inflammatory biomarkers. The extracts revealed also able to inhibit fungal and bacterial species involved in ulcerative colitis, including Candida albicans, Candida tropicalis, Staphylococcus aureus, and Staphylococcus thyphimurium. Finally, we also showed the antiproliferative effects exerted by the EA extracts on human colon cancer HCT116 cell line. Concluding, our results indicated that O. sipyleum extracts displayed promising therapeutic properties which warrants further validation. Practical applications The present phytochemical and biological studies, including antioxidant, anti‐inflammatory, and antimicrobic assessments, showed significant protective effects exerted by O. sipyleum extracts in an experimental model of ulcerative colitis. The results are intriguing and suggest potential applications O. sipyleum extracts as sources of natural agents for the management of clinical symptoms related to ulcerative colitis, characterized by increased burden of oxidative stress and microbiome dysbiosis.

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Section: Introductionmentioning

confidence: 91%

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

et al. 2019

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“…However, the continuous mobilization of these enzymes due to the daily introduction of free radical-generating polyherbs then led to the depletion of antioxidant enzymes as a result of exhaustion in the course of scavenging the overproduced free radicals. The scavenging activities of these enzymes are needed so as to prevent the deleterious effects of free radicals, and as well inhibit further oxidation of cellular components such as proteins, nucleic acids and lipids by molecular oxygen [23].…”

Section: Discussionmentioning

confidence: 99%

Depletion of Hepatic Antioxidant Enzymes in Experimental Albino Rats Due to Polyherbal Medicines Administration

Emaleku¹

2018

J Stem Cell Res Ther

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Objective: Plants and their derived products have served as veritable sources of foods and medicines for humans and animals from the outset, and the emergency of polyherbs (plants-derived products) in recent years has received the widest publicity and patronage by the Nigerian population as alternative medicines. It is not over statement to say many of them lack empirical data or validation to support the upsurge and prevalence in their usage as folk medicines, and little or no scientific data exist on their potential side effects. This study therefore investigates "the effects of some of these polyherbal drugs on hepatic antioxidants in experimental rats". Methods: Eight of the nine groups containing five animals each used for this study were administered eight different polyherbal drugs following different manufacturer's recommended dosage, while the ninth group served as control with no polyherb treatment. The study lasted for seven weeks i.e. forty-nine days, and on the 50th day, the animals were sacrificed after 12 h of previous overnight fasting, and their livers were excised for antioxidant assays. Results: Fidson bitters and Asheitu Adams blood purifier (ABP) significantly decreased superoxide oxidase (SOD) and glutathione-S-transferase (GST), while Yoyo bitter and Asheitu Adams formula for diabetes (AD) mostly decreased reduced glutathione (GSH) in a non-significant manner at (p<0.05). Conclusion: All polyherbal medicines caused depletion of hepatic antioxidant enzymes (SOD and GST), which is an indication of oxidative stress condition, but some of them improved non-enzymatic antioxidants like malondialdehyde (MDA) and vitamin C.

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“…The degree of conversion is indicated with the decrease in the absorbance. A good antioxidant property is to readily donate hydrogen ions; hence DPPH radical scavenging activity is a better method for screening the antioxidant properties 26 .…”

Section: Discussionmentioning

confidence: 99%

Untitled

2018

IJPSR

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The present study is designed to evaluate the in vitro antioxidant and in-vivo anti-inflammatory activities of Polyalthia longifolia (Sonn.) Thwaite seeds. Initially, the extracts (Nonpolar to polar) were screened for in-vitro antioxidant activity by DPPH, nitric oxide radical, hydroxyl radical, and superoxide radical considering ascorbic acid as standard. Among the extracts the ethyl acetate and methanolic extracts showed greater antioxidant activity further, the antioxidant activity of the extracts is supported by evaluating the total phenol content (114±1.7, 146.5±2.4 mg/g gallic acid equivalents respectively) and flavonoid content (2.86±0.10, 6.2±0.12 mg/g rutin equivalents respectively). Based on the initial study, the ethyl acetate and methanol extracts were evaluated for acute anti-inflammatory activity against the egg albumin (1%) induced inflammation. The selected extracts (200, 400 mg/kg) were administered 1 hour prior to the induction of inflammation. The anti-inflammatory activity is measured as a decrease in paw edema, measured at regular intervals (0.5, 1, 2, 3, 4, 5 h) of time, taking diclofenac (5 mg/kg) as a standard drug. Both the extracts showed significant dose-dependent anti-inflammatory activity when compared with the control. The 400 mg/kg dose of methanol extract showed greater percentage of inhibition (58.46%) when compared with the diclofenac (56.92%). The anti-inflammatory activity of the extracts is attributed due to the presence of active constituents like phenols, flavonoids, terpenes, steroids, etc. Hence Polyalthia longifolia seeds can be used in developing the lead molecules for the treatment of various chronic diseases like cancer, which are associated with the generation of ROS and inflammation.

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In vitroevaluation of antioxidant activity of some plant methanol extracts

Cited by 38 publications

References 20 publications

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

Depletion of Hepatic Antioxidant Enzymes in Experimental Albino Rats Due to Polyherbal Medicines Administration

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