Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

Abstract

The phytochemical, antiradical, and enzyme inhibition profile of three solvent extracts (ethyl acetate, methanol, water) of Origanum sipyleum were assessed. We also performed a pharmacological study in order to explore protective effects induced by extracts in inflamed colon. LC‐MS analysis revealed that the extracts contained different classes of phenolics. The aqueous extract showed the highest antioxidant and acetylcholinesterase (AChE) inhibitory effects. Total phenol and flavonoid contents were highest in aqueous and ethyl acetate extract, respectively. All extracts were effective in reducing colon pro‐oxidant and pro‐inflammatory biomarkers. The extracts revealed also able to inhibit fungal and bacterial species involved in ulcerative colitis, including Candida albicans, Candida tropicalis, Staphylococcus aureus, and Staphylococcus thyphimurium. Finally, we also showed the antiproliferative effects exerted by the EA extracts on human colon cancer HCT116 cell line. Concluding, our results indicated that O. sipyleum extracts displayed promising therapeutic properties which warrants further validation. Practical applications The present phytochemical and biological studies, including antioxidant, anti‐inflammatory, and antimicrobic assessments, showed significant protective effects exerted by O. sipyleum extracts in an experimental model of ulcerative colitis. The results are intriguing and suggest potential applications O. sipyleum extracts as sources of natural agents for the management of clinical symptoms related to ulcerative colitis, characterized by increased burden of oxidative stress and microbiome dysbiosis.

Bridelia speciosa Müll.Arg. Stem bark Extracts as a Potential Biomedicine: From Tropical Western Africa to the Pharmacy Shelf

“…Nevertheless, the methanol extract of B. speciosa displayed anti-mycotic activity on C. albicans (YEPGA 6379) that deserves further investigation. This inhibitory effect is consistent with its major content in total phenolic compounds [24,25], as confirmed by both colorimetric assays (Table 1) and independent HPLC-fluorimetric analysis ( Table 7). While considering both the incidence of C. albicans opportunistic infections occurring in liver disorders [50] and the traditional use of Bridelia genus [9], a pharmacological investigation was subsequently performed to explore anti-proliferative effects against the liver cancer HepG2 cell line and protective effects on isolated rat liver specimens challenged with the LPS pro-inflammatory stimulus.…”

“…Antibiotic and antimycotic assays were performed according to our previous studies [24,25]. The detailed description is reported in the Supplementary Material.…”

A GC–MS method validation for quantitative investigation of some chemical markers inSalvia hypargeiaFisch. & C.A. Mey. of Turkey: Enzyme inhibitory potential of ferruginol

“…In recent years, phenolic acids have been a focus of interest by food producers and researchers owing to their use in the human diet, their notable antioxidant activities, and their role in the prevention of oxidative stress-induced diseases (Yener et al, 2018;Ertas et al, 2014;. Many scientific reports have been published on the determination of plant components by LC-MS/MS technique (Yilmaz et al, 2018;Zengin et al, 2018Zengin et al, , 2019. In this study, phenolic contents of ethanol extracts prepared from various parts of S.…”

An investigation of chemical content, enzyme inhibitory propert, antioxidant and antibacterial activity of Aristolochia bodamae Dingler (develiotu) (Aristolochiaceae) root extracts from Samsun, Turkey

“…Chemical and biochemical activities of many plants were found in vivo and in vitro conditions. [ [5][6][7][8]10 ]…”

“…All over the world and in Turkey is increasing interest in treatment with herbal medicine. [ [4][5][6][7][8][9] Instead of synthetic drugs, humanity has preferred natural antioxidant plants and very important roles in removing damage caused by free radicals.…”