Abstract:
Background: Intestinal parasites are among the most important infectious agents with an impact on human health. Indeed, in the lack of an available treatment option, these parasites could constitute a real health problem in the population.
Aim: In the present study, we investigated the effect of a synthetic heterocyclic compound on two intestinal parasites (Entamoeda histolytica and Gardia lamblia).
Methods: The parasite isolates tested were collected from outpatients at the General Pediatric Hospital in Kirkuk, Iraq, between September 2019 and January 2020. Thus, we studied the in vivo and in vitro pharmaceutical activity of the ingredient on both parasites. The toxicological effects of the substance on some blood parameters and liver and kidney function tests were also studied.
Results: After five days of treatment, the drug's in vivo action on G. lamblia resulted in an inhibition rate of 88.2 percent at a dose of 1 mg/kg. On the other hand, we observed that the influence of this synthetic substance on cultured E. histolytica was very close to the metronidazole effect. The maximum result was at a concentration of 1 g/ml and was obtained after 72 hours of incubation with an inhibition rate of 89.4%. The substance did not affect the blood parameters or the studied liver and kidney functions.
Conclusion: It can be concluded that this substance is highly effective against both E. histolytica and G. lamblia, and that it has no toxic effects on the studied parameters. Therefore, it could be a promising pharmacophore for intestinal protozoan parasites including E. histolytica and G. lamblia and an alternative or competitor to the current medications available.
A chemo-enzymatic study of the enzyme G-6-PhD in the blood of people with leukemia and lipid level estimation and blood tests for cancer patients in the Kirkuk city .It included 80 samples, 30 of them are healthy as a control group and 50 patients with leukemia, which was collected from the center of cancer tumors in Kirkuk and the ages (1 to 60) years old. During this study was evaluated the level of effectiveness of G-6-PhD from red blood cells by using the diagnostic kit (BioLaBo). There is a decrease in the level of G-6-PhD enzyme effectiveness, especially in the second age group where there was a clear decrease in this category compared to healthy people. In addition, serum lipid levels were measured for people with leukemia, where they found a decrease in fat levels (HDL , CHOL) and an increase of (V.LDL , LDL ,T.G). Also, some blood tests were conducted in people with leukemia. There was a decrease in the level of (HB , PLT , PCV) and increase at the level of both (WBC) compared to healthy.
In this research, the kinetics study of one isoenzyme of Maltase, which purified from the urine of diabetes mellitus patients was studied. Maltase isoenzyme was obeyed Michaelis-Mentons eqution. The optimum concentration of their substrate Maltose was (300 mmol/dm 3). Their Km values was determined. Isoenzyme of Maltase have shown an optimum pH at (6.5) for phosphate buffer and (7.5) for tri ethanol amine. Maltase isoenzyme obeyed Arrhenius eqution up to 25°C and their Ea and Q 10 constant were determined, after measuring the activity of Maltase of urine of diabetes mellitus patients. Isoenzyme of Maltase was separated and purified in previous study from urine of patients of diabetes mellitus.
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