Drought is a major constrain in crop production that reduce growth and cause yield loss of up to 70%. Transcription factor plays a major role in cellular regulation and physical changes of plants as a response to stress. A number of transcription factors, such as CBF/DREB, NAC, zinc finger protein are regulators during stress. The Oryza sativa NAC6 (OsNAC6) gene is one of the transcription factor in rice that can regulate gene expression during stress conditions. Thus, pCambia 1305 harboring OsNAC6 chimaeric gene with CaMV 35S promoter was introduced into rice zygotic embryo using Agrobacterium tumefaciens mediated transformation to regenerate transgenic rice overexpressing the transgene. As many as 39 putative transgenic lines in which 21 lines possitively harbored hpt gene have been regenerated. The positive identification of hpt in the regenerated transgenic rice indirectly indicated integration of the targeted OsNAC6 since both transgenes were part of the same T-DNA. Further analysis indicated the presence of 1-3 copies of transgene integration in the genome. The expression of OsNAC6 transgene in the transgenic rice line#C.73, C.83 and C.91 were higher than wild type non-transgenic one. Further analysis indicated those three transgenic lines carrying OsNAC6 transgene exhibited higher tolerance against drought and salinity stresses. Moreover, three known stress-associated regulatory genes (AP2, Zincfinger protein and MYB) were up-regulated in those three transgenic lines. These findings demonstrated that OsNAC6 might be a candidate of stress-responsive NAC regulatory gene that can be used to develop either drought or salt tolerant tolerant transgenic plants.
The Saccharomyces cerevisiae Yvh1, a dual-specificity protein phosphatase involved in glycogen accumulation and sporulation, is required for normal vegetative growth. To further elucidate the role of Yvh1, we generated dominant mutants suppressing the slow growth caused by YVH1 disruption. One of the mutant alleles, designated as SVH1-1 (suppressor of Δyvh1 deletion), was identical to MRT4 (mRNA turnover) that contained a single-base substitution causing an amino acid change from Gly(68) to Asp. Mrt4(G68D) restored the deficiencies in growth and rRNA biogenesis that occurs in absence of Yvh1. Here, we report that the interaction between Mrt4 and Yvh1 is also essential for normal glycogen accumulation and mRNA decay as well as the induction of sporulation genes IME2, SPO13 and HOP1. The Mrt4(G68D) could restore the plethora of phenotypes we observed in absence of Yvh1. We found that Yvh1 is not essential for wild-type induction of the transcriptional regulator of these genes, IME1, suggesting that either translation or post-translational modification to activate Ime1 has been compromised. Since a defect in ribosome biogenesis in general can be related to other various defects, the ribosome biogenesis defect caused by absence of Yvh1 might be an indirect cause of observed phenotypes.
The current study makes use of life cycle assessment to evaluate the potential greenhouse gas (GHG) savings in coal electricity generation by 5% co-firing with sorghum pellets. The research models the utilization of 100 thousand hectares of under-utilized marginal land in Flores (Indonesia) for biomass sorghum cultivation. Based on equivalent energy content, 1.12 tons of pellets can substitute one ton of coal. The calculated fossil energy ratio of the pellets was 5.8, indicating that the production of pellets for fuel is energetically feasible. Based on a biomass yield of 48 ton/ha•yr, 4.8 million tons of pellets can be produced annually. In comparison with a coal system, the combustion of only pellets to generate 8,300 GWh of electricity can reduce global warming impacts by 7.9 million tons of CO2-eq, which is equivalent to an 85% reduction in GHG emissions. However, these results changed when reduced biomass yield of 24 ton/ha•yr, biomass loss, field emissions, and incomplete combustion were considered in the model. A sensitivity analysis of the above factors showed that the potential GHG savings could decrease from the initially projected 85% to as low as 70%. Overall, the production of sorghum pellets in Flores and their utilization for electricity generation can significantly reduce the reliance on fossil fuels and contribute to climate change mitigation. Some limitations to these conclusions were also discussed herein. The results of this scenario study can assist the Indonesian government in exploring the potential utilization of marginal land for bioenergy development, both in Indonesia and beyond.
Genome- or gene-editing (abbreviated here as ‘GEd’) presents great opportunities for crop improvement. This is especially so for the countries in the Asia-Pacific region, which is home to more than half of the world’s growing population. A brief description of the science of gene-editing is provided with examples of GEd products. For the benefits of GEd technologies to be realized, international policy and regulatory environments must be clarified, otherwise non-tariff trade barriers will result. The status of regulations that relate to GEd crop products in Asian countries and Australasia are described, together with relevant definitions and responsible regulatory bodies. The regulatory landscape is changing rapidly: in some countries, the regulations are clear, in others they are developing, and some countries have yet to develop appropriate policies. There is clearly a need for the harmonization or alignment of GEd regulations in the region: this will promote the path-to-market and enable the benefits of GEd technologies to reach the end-users.
This research observes and analyses the agronomic characteristics and yield component of six T8 generation of Rojolele transgenic rice lines (P8, Q20, U10, W3, X22, Y7) harboring Cry1B::Cry1Aa gene fusion conferring resistance to yellow stem borer (Schirphopaga interculas) compared to the nontransgenic Rojolele control plants. The experiment was done in a biosafety containment at the Research Centre for Biotechnology-LIPI in a (4.0 x 3.2) m2 pot with 50 cm spacing amongst lines and 25 cm between plants of the same line with 10 replicates each line. PCR analysis using Cry1B::Cry1Aa specific primer showed that all lines tested were transgenic as shown by the presence of 816 bp amplicon. The W3 transgenic line had the most similar agronomic characteristics to the Rojolele control plants in terms of plant height, leaf length, tiller numbers, stem diameter and flowering time. Yield components of the W3 line such as panicle length and total grains were also the closest to the Rojolele control plants compared to other transgenic lines. However, the number of empty grains was the highest. In general, the agronomic characteristics and yield component of all transgenic lines were less than of the Rojolele control plants.
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