Agronomic characteristics and yield component of Rojolele transgenic rice resistant to Scirpophaga incertulas in biosafety containment

Abstract

This research observes and analyses the agronomic characteristics and yield component of six T8 generation of Rojolele transgenic rice lines (P8, Q20, U10, W3, X22, Y7) harboring Cry1B::Cry1Aa gene fusion conferring resistance to yellow stem borer (Schirphopaga interculas) compared to the nontransgenic Rojolele control plants. The experiment was done in a biosafety containment at the Research Centre for Biotechnology-LIPI in a (4.0 x 3.2) m2 pot with 50 cm spacing amongst lines and 25 cm between plants of the same line with 10 replicates each line. PCR analysis using Cry1B::Cry1Aa specific primer showed that all lines tested were transgenic as shown by the presence of 816 bp amplicon. The W3 transgenic line had the most similar agronomic characteristics to the Rojolele control plants in terms of plant height, leaf length, tiller numbers, stem diameter and flowering time. Yield components of the W3 line such as panicle length and total grains were also the closest to the Rojolele control plants compared to other transgenic lines. However, the number of empty grains was the highest. In general, the agronomic characteristics and yield component of all transgenic lines were less than of the Rojolele control plants.

Rojolele: a Premium Aromatic Rice Variety in Indonesia

“…By inserting Cry1B::Cry1Aa, transgenic line of Rojolele can produce crystal protein (cry) that have insecticidal action against S. incertulas [42]. Transgenic line of Rojolele which resistant to yellow stem borer has developed by the Research Center for Biotechnology in Indonesia and developing six T7 generation with stable inheritance [43]. These six lines were P8, Q20, U10, W3, X22, and Y7.…”

“…* Means followed by the different letters in a column are significantly different (P ≤ 0.05). Grain yield components of wild type and six transgenic lines of Rojolele[43] Means followed by the same letters in a column are not significantly different (P ≤ 0.05).…”

Rojolele: A Premium Aromatic Rice Variety in Indonesia

“…By inserting Cry1B::Cry1Aa, transgenic line of Rojolele can produce crystal protein (cry) that have insecticidal action against S. incertulas (Hofte and Whiteley, 1989). Transgenic line of Rojolele which resistant to yellow stem borer has developed by the Research Center for Biotechnology in Indonesia and developing six T7 generation with stable inheritance (Chairunisa and Nugroho, 2020). These six lines were P8, Q20, U10, W3, X22, and Y7.…”

“…Moreover, Nugroho et al (2020)described that the transgene is stable inheritance until T10 generation of the six transgenic lines. All of the transgenic lines showed less in agronomic Source: Chairunisa and Nugroho (2020) characteristics and grain yield components compared to Rojolele wild type (Tables 6 and 7). This is because the transgene Cry1B::Cry1 might be inserted in a region where the growth regulation present which affects the plant development.…”

Agronomic characteristics and genetic relationship of putative transgenic rice lines of cv. Fatmawati with the Glu-1Dx5 transgene

“…Swarna derived from marker-assisted backcrossing of GR2-R1 (Golden Rice-R1) event, showing dwarf with pale green leaves and short panicle length, low grain number and yield. More recently, Chairunisa et al (2020) found that plant height, leaf length and width, stem diameter, tiller number and productive tiller numbers of six transgenic rice lines that they produced are generally less than those of cv. Rojolele (wild-type).…”