Resveratrol has a
neuroprotective effect against cerebral ischemia. The objective of this study was to
identify proteins that are differentially expressed in the cerebral cortex of vehicle- and
resveratrol-treated animals during ischemic injury. Focal cerebral ischemia was induced as
middle cerebral artery occlusion (MCAO) in male rats. Rats were treated with vehicle or
resveratrol before MCAO, and cerebral cortex was collected 24 hr after MCAO. Cerebral
cortex proteins were identified by two-dimensional gel electrophoresis and mass
spectrometry. Several proteins were identified as differentially expressed between
vehicle- and resveratrol-treated animals. Among these proteins, expression of
peroxiredoxin-5, isocitrate dehydrogenase [NAD+], apolipoprotein A-I and
ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group,
whereas resveratrol attenuated the injury-induced decrease in expression of these
proteins. However, expression of collapsing response mediator protein 2 was increased in
the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in
the expression of this protein. These findings suggest that resveratrol modulates the
expression of various proteins that associated with oxidative stress and energy metabolism
in focal cerebral ischemia.
Ginkgo biloba extract (EGb 761) exerts a neuroprotective effect against ischemic brain injury through an anti-apoptotic mechanism. Parvalbumin is a calcium buffering protein that plays an important role in modulating intracellular calcium concentration and regulating apoptotic cell death. The aim of this study was to investigate whether EGb 761 affects parvalbumin expression in cerebral ischemic injury. Adult male Sprague-Dawley rats were treated with vehicle or EGb 761 (100 mg/kg) prior to middle cerebral artery occlusion (MCAO) and cerebral cortex tissues were collected 24 h after MCAO. A proteomic approach revealed a reduction in parvalbumin expression in the vehicle-treated animals, whereas EGb 761 pretreatment attenuates the ischemic injury-induced decrease in parvalbumin expression. RT-PCR and Western blot analyses clearly confirmed the fact that EGb 761 prevents the injury-induced decrease in parvalbumin. Moreover, the results of immunohistochemical staining showed that the number of parvalbumin-positive cells was lower in vehicle-treated animals than in sham-operated animals, and EGb 761 averted this decrease. Thus, these results suggest that the maintenance of parvalbumin expression is associated with the neuroprotective function of EGb 761 against neuronal damage induced by ischemia.
Testicular torsion is a urological emergency that leads to serious testicular
damage and male infertility. We performed this study to identify specific proteins that
are differentially expressed in response to testicular torsion and detorsion-induced
ischemia-reperfusion (I-R) injury. Adult male rats were divided into two groups: a
sham-operated group and a testicular I-R group. Testicular torsion was induced by rotating
the left testis 720° in a clockwise direction for 1 hr, and then, detorsion was performed
for 24 hr. After this testicular tissues were collected, protein analysis was performed
using two-dimensional gel electrophoresis and Western blot analyses. Testicular I-R injury
resulted in serious histopathologic damage to the germinal cells in the seminiferous
tubules and increased the number of TUNEL-positive cells in testicular tissue. Specific
protein spots with a greater than 2.5-fold change in intensity between the sham-operated
and testicular I-R groups were identified by mass spectrometry. Among these proteins,
levels of peroxiredoxin 6, thioredoxin, heterogeneous nuclear ribonucleoproteins,
ubiquitin carboxyl terminal hydrolase isozyme L5 and zinc finger AN1-type domain 3 were
decreased in the testicular I-R group compared to the sham-operated group. Moreover,
Western blot analysis clearly showed the decrease of these proteins in the testicular I-R
group. These proteins have spermatogenesis and anti-oxidative functions. These findings
suggest that testicular I-R results in cell death due to altered expression of several
proteins with spermatogenesis and anti-oxidation functions.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.