Proteomic Identification of Proteins Differentially Expressed in Response to Resveratrol Treatment in Middle Cerebral Artery Occlusion Stroke Model

Abstract

Resveratrol has a neuroprotective effect against cerebral ischemia. The objective of this study was to identify proteins that are differentially expressed in the cerebral cortex of vehicle- and resveratrol-treated animals during ischemic injury. Focal cerebral ischemia was induced as middle cerebral artery occlusion (MCAO) in male rats. Rats were treated with vehicle or resveratrol before MCAO, and cerebral cortex was collected 24 hr after MCAO. Cerebral cortex proteins were identified by two-dimensional gel electrophoresis and mass spectrometry. Several proteins were identified as differentially expressed between vehicle- and resveratrol-treated animals. Among these proteins, expression of peroxiredoxin-5, isocitrate dehydrogenase [NAD+], apolipoprotein A-I and ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group, whereas resveratrol attenuated the injury-induced decrease in expression of these proteins. However, expression of collapsing response mediator protein 2 was increased in the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in the expression of this protein. These findings suggest that resveratrol modulates the expression of various proteins that associated with oxidative stress and energy metabolism in focal cerebral ischemia.

The Plant-Derived Compound Resveratrol in Brain Cancer: A Review

“…IDH1 mutations were predominantly found in human low-grade astrocytoma, oligodendroglioma, and secondary GBM. On the other hand, IDH2 mutations occurred less frequently in gliomas and were mutually exclusive of IDH1 mutations [119]. RES has been found to maintain IDH levels in a middle cerebral artery occlusion stroke model [120] and to protect the left ventricle by increasing IDH activity in myocardial infarction [121].…”

The neuroprotective effects of resveratrol preconditioning in transient global cerebral ischemia-reperfusion in mice

“…In addition, resveratrol protects rat brain from cerebral ischemic injury via a sirtuin 1-uncoupling protein 2 and TRPC6/CREB pathways (7,16). Resveratrol also regulates the expression of various proteins associated with oxidative stress and energy metabolism in focal cerebral ischemia (25). Furthermore, resveratrol can enhance the neuroprotection when coadministrated with lipoic acid in cerebral ischemia (24).…”

Resveratrol modulates the Akt/GSK-3β signaling pathway in a middle cerebral artery occlusion animal model

“…Resveratrol alleviated MCAO-induced neurological behavior deficits. Moreover, our previous study has been shown that resveratrol regulates the expression of various proteins that associated with oxidative stress in focal cerebral ischemia [26].…”

“…Animals were divided into the following four groups: vehicle + sham, resveratrol + sham, vehicle + MCAO, and resveratrol + MCAO (8 rats per group). Resveratrol (30 mg/kg, Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 0.05% dimethyl sulfoxide (DMSO; Sigma-Aldrich) in phosphate buffered saline and immediately intraperitoneally injected [26]. Vehicle-treated animals were injected with only DMSO solution without resveratrol.…”