Resveratrol has a
neuroprotective effect against cerebral ischemia. The objective of this study was to
identify proteins that are differentially expressed in the cerebral cortex of vehicle- and
resveratrol-treated animals during ischemic injury. Focal cerebral ischemia was induced as
middle cerebral artery occlusion (MCAO) in male rats. Rats were treated with vehicle or
resveratrol before MCAO, and cerebral cortex was collected 24 hr after MCAO. Cerebral
cortex proteins were identified by two-dimensional gel electrophoresis and mass
spectrometry. Several proteins were identified as differentially expressed between
vehicle- and resveratrol-treated animals. Among these proteins, expression of
peroxiredoxin-5, isocitrate dehydrogenase [NAD+], apolipoprotein A-I and
ubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group,
whereas resveratrol attenuated the injury-induced decrease in expression of these
proteins. However, expression of collapsing response mediator protein 2 was increased in
the vehicle-treated group, whereas resveratrol prevented the injury-induced increase in
the expression of this protein. These findings suggest that resveratrol modulates the
expression of various proteins that associated with oxidative stress and energy metabolism
in focal cerebral ischemia.
Mesenchymal stem cells (MSCs) are pluripotent adult stem cells. It has been shown that MSCs secrete neurotrophic factors involving nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Also, these neurotrophic factors can upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells and neural stem cells. Here, we investigated the effect of co-culturing rat E13.5 ventral mesencephalic cells (VMCs) with MSCs from rat bone marrow on TH expression and dopamine (DA) content. The study consisted of 3 groups: MSC, VMC and a combined MSC+VMC group. All groups were cultured in serum-free neuro-basal medium for 3 days. Thereafter, each group was analyzed by RT-PCR, western blotting, and HPLC. The co-culture group showed a higher expression at TH and DA than the VMC group. However, TH and DA were not present in the MSC group. These observations suggest that MSCs could be an alternative source for treating neurodegenerative diseases such as Parkinson's disease (PD).
ABSTRACT. Ethanol exposure is known to suppress male reproductive activity in laboratory animals and humans. The present study was designed to evaluate whether chronic ethanol exposure decreases proliferative activity or increases apoptosis in the testes. Ethanol (1.5 g/kg or 3 g/kg i.p., 15% v/v in saline) was administrated to adult male rats for 10 days. Proliferating cell nuclear antigen (PCNA) was used as a proliferative marker. Western blot analysis showed that ethanol administration significantly reduced the level of PCNA. Also, immunoreactivity of PCNA-positive cells in the spermatogonia and primary spermatocytes were decreased by ethanol exposure. However, the number of TUNEL-positive cells was significantly increased in the testicular germ cells of ethanol-treated rats. Moreover, ethanol administration significantly increased the level of activated caspase-3 in testes. In conclusion, our findings suggest that ethanol may partly contribute to the suppression of male reproductive activity through a reduction of cell proliferation and an enhancement of cell death in rat testes.
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