Common variants among genes coding for enzymes in sex steroid biosynthetic pathways may influence the risk of endometriosis in Iraqi women patients in the last years. Cytochrome P450c17a1 (CYP17), a gene that codes for a key enzyme (cytochrome P450c17a1) in a rate-limiting step of estrogen biosynthesis has attracted considerable attention as an important gene for endometriosis. To evaluate the relationship between common genetic variations in CYP17 and endometriosis risk and determine the main effects of those variations on the gene expression. A women-based case control study of Iraqi women aged range (23–46), the associations between selected single-nucleotide polymorphisms (SNPs) in the CYP17 gene and endometriosis diagnosis in fifty women and thirty disease-free controls were evaluated. The study found a significant association (P ≤ 0.01)between endometriosis and selected SNPs of CYP17 gene, with the homozygous genotype conferring decreased risk. A highly significant difference (P ≤ 0.01) in CYP17 gene expression from women with versus without endometriosis and increased by 1.56-fold in women with endometriosis. These findings suggest that variation in or around CYP17 may be associated with endometriosis development in the Iraqi women.
Breast Cancer is caused by malignant tissue cells and has become one of the world’s biggest medical problems. The goal of this research was to determine the serum concentration of markers of oxidative stress that play an important role in the multiple factors involved in breast cancer development, growth, and invasion. Serum of 90 women patients (45 breast cancer and 45 benign breast tumors) and 42 healthy individuals as control group were used in this study. Serum level of MDA, PC, 8-OHdG, IL-1β and Caspase-3 were measured by ELISA. Highly significantly increased levels (p<0.01) of MDA, PC, 8-OHdG, IL-1β and Caspase-3 were found in breast cancer and benign breast tumor when compared to healthy controls. The MDA and Caspase-3 level are considered strong parameters to diagnose and detection for breast cancer using the ROC curve. High lipid peroxidation is a significant risk factor for breast cancer and the activation of apoptosis and pro-inflammatory activity may be due to elevated levels of IL-1β & Caspase-3 in breast cancer cells. Nonetheless, lipid peroxidation and Caspase-3 are major factors in breast cancer growth and progression.
Growing research has shown that multiple sclerosis (MS) patients have lower levels of 25-OHvitD3 than healthy controls. The purpose of this study was to evaluate the serum concentration of 25-OHvitD3, CYP24Alenzyme in MS patients and calcium-phosphate metabolism indices depending on the different disease lines and gender. Furthermore, we elevated the relationship between study parameters and gender with degree of motor disability according to the Expanded Disability Status Scale (EDSS). Serum of 110 Iraqi MS patients (50 male and 60 female) and 63 healthy individuals (30 male and 33 female) as control groups were used in our study. Serum level of 25-OHvitD3, CYP24A1and PTH were measured by ELISA. Serum level for calcium, phosphorus and magnesium were performed with a BioSystems A25 and A15 analyzers. Significantly lower level of CYP24A1 and 25-OHvitD3 was found in MS patients when compared to control groups. The Levels of CYP24A1 and 25-OHvitD3 were positively correlated in female and significantly decreased with EDSS in female groups. Also, in MS patients a highly significant decrease in calcium, a considerable increase in level of PTH, phosphorous and magnesium. In this study the negative correlation between 25-OHvitD3 and the degree of motor disability, according to EDSS in MS patients was confirmed. We identified a non-significant difference in the level of 25-OHvitD3 regarding on gender. Still, we confirmed the association between gender and MS disease development regarding the levels of 25-OHvitD3, PTH and phosphorus.
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