In this study we designed a cost-effective solar photovoltaic (PV) powered reverse osmosis (RO) desalination plant for Masdar Institute of Science and Technology. The proposed system allowed us to design a RO plant that does not rely on expensive batteries or extra land and drastically decreased the government expenses to subsidize the water production cost to 84% of the current expenses. In additional, the system allowed to reduce the emission of GHG by 1,035 tCO2 annually. The equity payback time was found to be 23.3 years and the benefit-cost ratio to be 0.72. Comparing the results obtained with the conventional values, it can well be said that this system would definitely provide a much needed efficient alternative to the current method of water purification making it more sustainable and economically feasible at the same time.

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Antimicrobial resistance (AMR) is a global public health issue, and the rise of carbapenem-resistant bacteria needs attention. While progress is being made in the rapid detection of resistant bacteria, affordability and simplicity of detection still need to be addressed. This paper presents a nanoparticle-based plasmonic biosensor for detecting the carbapenemase-producing bacteria, particularly the beta-lactam Klebsiella pneumoniae carbapenemase (blaKPC) gene. The biosensor used dextrin-coated gold nanoparticles (GNPs) and an oligonucleotide probe specific to blaKPC to detect the target DNA in the sample within 30 min. The GNP-based plasmonic biosensor was tested in 47 bacterial isolates: 14 KPC-producing target bacteria and 33 non-target bacteria. The stability of GNPs, confirmed by the maintenance of their red appearance, indicated the presence of target DNA due to probe-binding and GNP protection. The absence of target DNA was indicated by the agglomeration of GNPs, corresponding to a color change from red to blue or purple. The plasmonic detection was quantified with absorbance spectra measurements. The biosensor successfully detected and differentiated the target from non-target samples with a detection limit of 2.5 ng/μL, equivalent to ~103 CFU/mL. The diagnostic sensitivity and specificity were found to be 79% and 97%, respectively. The GNP plasmonic biosensor is simple, rapid, and cost-effective in detecting blaKPC-positive bacteria.