Lamtoro (Leucaena leucocephala cv Tarramba) merupakan salah satu hjauan pakan tenak yang potensialuntuk dikembangkan. Perbanyakan vegetatif dengan stek batang merupakan salah satu alternatif perbanyakanyang memiliki keunggulan dapat dilakukan secara cepat oleh masyarakat dan memliki sifat yang sama denganinduknya. Tujuan penelitian ini adalah untuk mengetahui umur fisiologis dan jenis zat pengatur tumbuhyang terbaik untuk keberhasilan stek tanaman lamtoro. Penelitian dilakukan menggunakan rancangan acakkelompok dua faktor perlakuan dan diulang tiga kali. Faktor pertama umur fisilogis batang terdiri atas tigataraf yaitu pangkal, tengah dan bagian atas (ujung) batang. Faktor kedua penggunaan zat pengatur tumbuhyang digunakan yang terdiri dari 4 taraf, yaitu: tanpa zat pengatur tumbuh, indole-3-butyric acid (IBA),?-naphtaleneacetic acid (NAA) dan root up. Hasil penelitian menunjukkan umur fisiologis batang dan zatpengatur tumbuh berpengaruh nyata terhadap keberhasilan stek lamtoro, namun interaksi antara keduafaktor perlakuan tidak terdapat pengaruh nyata terhadap semua peubah yang diamati. Keberhasilan stek daribatang bagian pangkal (88,10%) lebih tinggi dibandingkan batang bagian tengah dan ujung masing-masing82,14% dan 35,71%. Penggunaan zat pengatur tumbuh IBA, NAA dan Root Up memberikan persentase steklamtoro hidup yang lebih tinggi dibandingkan tanpa penggunaan zat pengatur Kata kunci: auksin, leguminosa, Leucaena leucocephala cv Tarramba, perbanyakan vegetatif, umurfisiologis
Halotolerant bacteria are reported as a potential biostimulant to mitigate saline stress on various crops. The mechanism of halotolerant bacteria in elevating plant growth under saline stress is associated with their plant growth-promoting (PGP) traits. This study evaluated the activity of single-strain halotolerant phosphate-solubilizing bacteria and its consortia with halotolerant bacteria that were able to fix nitrogen and produce ACC deaminase in alleviating salinity stress on Zea mays seedlings under in vitro conditions. One single strain and five consortia of bacterial isolates were tested for seedling growth assay under four salinity levels (0, 60, 80, and 100 mM). In vitro assay showed that halotolerant bacteria B1 (Vibrio alginolycticus) and all consortia significantly increased root number at 60 mM salinity level. Consortium B3 (V. alginolyticus+Salinicola zeshunii) was also able to improve the fresh weight of seedlings significantly (by 63.3%). Moreover, inoculation of consortium B3 affected more proline and soluble sugar accumulation in Z. mays seedlings when compared to uninoculated seedlings. We conclude that the application of a consortium of halotolerant bacteria V. alginolyticus+S. zeshunii was potentially used in improving Z. mays growth in slightly and moderately saline areas.
Bacterial endophyte and rhizobacteria were reported to associate with medicinal plants including Zingiberaceae plants and involved in growth promotion. These beneficial bacteria are promising candidates as biostimulants because of their ability in promoting plant growth. This study aims to evaluate the activity of endophytic and rhizosphere bacteria isolated from Curcuma xanthorrhiza (Javanese turmeric) in promoting rice seedling and Javanese turmeric growth. Fifty-seven of 150 total bacterial isolates with negative hemolysis and hypersensitivity reactions were characterized to investigate their plant growth-promoting (PGP) traits. Ten selected bacteria (two bacterial endophytes and eight rhizobacteria) with multiple PGP traits were inoculated to rice seed with seed treatment and inoculated to Javanese turmeric rhizome with seed treatment and seed treatment+soil soil drenching. Our results showed that bacterial isolates tested on rice seed promoted rice seedling growth significantly. A total of five, three, six, and three bacterial isolates could increase leaf number, root length, fresh shoot weight, and fresh root weight of rice seedling (p<0.05), respectively. In contrast, all of the bacterial isolates tested on Javanese turmeric rhizomes showed a non-significant effect on the plant growth. Further studies should be considered to investigate the effect of formulated potential bacterial isolates with different application frequencies and environmental conditions on the harvest yield of rice and Javanese turmeric as well as active compounds of Javanese turmeric.
Systematic management of oil palm-cattle integration considers as one of the best alternative solutions to meet the need of increasing local beef and dairy. The change in oil palm plantation management systems can influence the soil microbial community. Microbes mediate many key processes in soils and are essential in driving ecosystem stability and sustainability of oil palm production. The research aimed to investigate the influence of grazing in an oil palm plantation on the bacteria involved in the soil nitrogen cycle. We evaluated the abundance of diazotrophic, nitrifying, and denitrifying bacteria in the grazing and ungrazing area of oil palm plantations. Grazing area subject to 11 and 10 times cattle grazing rotation with the two-month interval between rotation were studied. The enumeration of bacteria was evaluated using the Most Probable Number (MPN) technique. Generally, diazotrophic bacteria, nitrifier, and denitrifier were higher in the grazing area compare to ungrazing area. However, the difference between that two grazing-plantation management is not significant (P>0.05). In summary, our results show that an addition of organic materials from the dung of cattle in the grazing area of oil palm plantation influences the soil microbial community including N-transforming bacteria
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