Taro or Satoimo (Colocasia esculenta (L) Schott var antiquorum) is an alternative of non-rice food. To support saitomo mass cultivation in several regions in Indonesia, shoot multiplication and induction of satoimo microtuber through in vitro technique is amongst the stage to be undertaken. The aims of this study were to determine the effect of BAP (benzylaminopurine) and sucrose for shoot multiplication and microtuber induction of in vitro culture of satoimo. The experiment was arranged in two factors: BAP (0, 1, 2 and 3 mg/L) and sucrose (30, 60, 90 and 120 g/L). The result showed that the single effect of BAP or sucrose and interaction of both significantly increased the number of shoots. The effect of 2 mg/L BAP was more homogeneous than that of 1 and 3 mg/L BAP. Sucrose with the concentration of 30 g/L was the best concentration for shoot multiplication. The highest number of microtuber was achieved with 2 mg/L BAP + 30 g/L sucrose treatments, but tended to decrease due to increasing sucrose concentration. In 2 and 3 mg/L BAP treatments, the number of microtuber increased along with the increasing sucrose concentration.Keywords: satoimo, in vitro shoot, microtuber, benzylaminopurine, sucrose ABSTRAKSatoimo (Colocasia esculenta (L) Schott var antiquorum) merupakan bahan pangan alternatif non-beras. Untuk mendukung produksi massal satoimo di beberapa wilayah di Indonesia, multiplikasi tunas dan induksi umbi mikro secara in vitro merupakan tahapan yang harus dilakukan. Tujuan penelitian ini adalah untuk mengetahui pengaruh BAP dan sukrosa terhadap multiplikasi tunas dan induksi umbi mikro satoimo dalam kultur in vitro. Perlakuan terdiri dari 4 taraf konsentrasi BAP (0, 1, 2 dan 3 mg/L) dan 4 taraf konsentrasi sukrosa (30, 60, 90 dan 120 g/L). Hasil penelitian menunjukkan bahwa BAP dan sukrosa secara tunggal serta interaksinya berpengaruh nyata terhadap multiplikasi tunas in vitro. Pengaruh konsentrasi BAP 2 mg/L lebih homogen dibandingkan perlakuan BAP 1 dan 3 mg/L. Sukrosa 30 g/L merupakan konsentrasi terbaik untuk multiplikasi tunas. Umbi mikro terbanyak terdapat pada perlakuan BAP 1 mg/L + sukrosa 30 g/L tetapi cenderung mengalami penurunan jika konsentrasi sukrosa dinaikkan pada konsentrasi BAP tetap. Pada perlakuan BAP 2 dan 3 mg/L jumlah umbi mikro yang terbentuk cenderung meningkat seiring dengan peningkatan konsentrasi sukrosa.Kata kunci: satoimo, tunas in vitro, umbi mikro, benzilaminopurin, sukrosa
Lamtoro (Leucaena leucocephala cv Tarramba) merupakan salah satu hjauan pakan tenak yang potensialuntuk dikembangkan. Perbanyakan vegetatif dengan stek batang merupakan salah satu alternatif perbanyakanyang memiliki keunggulan dapat dilakukan secara cepat oleh masyarakat dan memliki sifat yang sama denganinduknya. Tujuan penelitian ini adalah untuk mengetahui umur fisiologis dan jenis zat pengatur tumbuhyang terbaik untuk keberhasilan stek tanaman lamtoro. Penelitian dilakukan menggunakan rancangan acakkelompok dua faktor perlakuan dan diulang tiga kali. Faktor pertama umur fisilogis batang terdiri atas tigataraf yaitu pangkal, tengah dan bagian atas (ujung) batang. Faktor kedua penggunaan zat pengatur tumbuhyang digunakan yang terdiri dari 4 taraf, yaitu: tanpa zat pengatur tumbuh, indole-3-butyric acid (IBA),?-naphtaleneacetic acid (NAA) dan root up. Hasil penelitian menunjukkan umur fisiologis batang dan zatpengatur tumbuh berpengaruh nyata terhadap keberhasilan stek lamtoro, namun interaksi antara keduafaktor perlakuan tidak terdapat pengaruh nyata terhadap semua peubah yang diamati. Keberhasilan stek daribatang bagian pangkal (88,10%) lebih tinggi dibandingkan batang bagian tengah dan ujung masing-masing82,14% dan 35,71%. Penggunaan zat pengatur tumbuh IBA, NAA dan Root Up memberikan persentase steklamtoro hidup yang lebih tinggi dibandingkan tanpa penggunaan zat pengatur Kata kunci: auksin, leguminosa, Leucaena leucocephala cv Tarramba, perbanyakan vegetatif, umurfisiologis
Systematic management of oil palm-cattle integration considers as one of the best alternative solutions to meet the need of increasing local beef and dairy. The change in oil palm plantation management systems can influence the soil microbial community. Microbes mediate many key processes in soils and are essential in driving ecosystem stability and sustainability of oil palm production. The research aimed to investigate the influence of grazing in an oil palm plantation on the bacteria involved in the soil nitrogen cycle. We evaluated the abundance of diazotrophic, nitrifying, and denitrifying bacteria in the grazing and ungrazing area of oil palm plantations. Grazing area subject to 11 and 10 times cattle grazing rotation with the two-month interval between rotation were studied. The enumeration of bacteria was evaluated using the Most Probable Number (MPN) technique. Generally, diazotrophic bacteria, nitrifier, and denitrifier were higher in the grazing area compare to ungrazing area. However, the difference between that two grazing-plantation management is not significant (P>0.05). In summary, our results show that an addition of organic materials from the dung of cattle in the grazing area of oil palm plantation influences the soil microbial community including N-transforming bacteria
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