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Endophytic microbes are microbes which live symbiotically inside the plant tissue. The potential of those microbes were studied for many purposes such as industrial and agriculture. The purpose of this research was to study the potential of endophytic microbes for bioactive compound production as plants protection agent. From 238 bacteria isolates tested, it were identified that 44 bacteria were against the Xanthomonas campestris, 49 isolates against Pseudomonas solanacearum, 28 isolates against Colletotricum gloeosporioides, 18 isolates against Fusarium oxysporum, 19 isolates against Xanthomonas campestris and Pseudomonas solanacearum, 6 isolates against X. campestris and C. gloeosporioides, four isolates against X. campestris and F. oxysporum, two isolates against P. solanacearum and F. oxysporum, seven isolates against C. gloeosporioides and F. oxysporum, five isolates againt X. campestris, P. solanacearum and C. gloeosporioides. Fermentation process was conducted to collect the bioactive compound against pathogen. Isolates HL.50B.106 could produce bioactive compound against C. gloeosporioides, and F. oxysporum, isolates HL.13B.21 against X. campestris and P. solanacearum, isolates Hl.12B.19 against X. campestris, P. solanacearum and C. gloeosporioides. Thin layer chromatography analysis showed specific spot, different from standard (media). Spot was purple in color after sprayed with CeSO 4. Rf value of HL.50B.106 extract were 0.51 and 0.53 (fraction 1 and 2), 0.62 and 0.64 (fraction 3 and 4).

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Production of glucoamylase generally used through the fermentation-using microorganism. One of microorganism source which never been studied are from endophyte fungus. The purpose of this research is to study the potential microbes of Gunung Halimun Nasional Park (GHNP) for glucoamylase production. Thirty-seven isolate of endophyte fungi has been investigated for the ability of glucoamylase production on PSA (Potato starch agar) media with the strength of clear zone and colony. The potential isolates inoculated to Czapek media to produce glucoamylase on 50 ml scale and measured its activity every 24 hours of incubation time for 96 hours. The best isolate then was reproduced on the larger media (100 ml), and resumed with filtration and ultra-filtration. The enzyme activity, specific activity, and degree of protein was measured in every phase. Selection of amylolitic strength resulted that HL.110F.488 produced the highest amylolitic strength with halo size of 10.47 cm2, or equal with hydrolysis of starch of 0.0494 gram for 96 hours, while both isolate HL.44F.199 and HL.45F.205 had low amylolitic capacities but a very wide of colony growth, each 38.54 cm2 and 30.76 cm2. Isolat HL.44F.199 produced the highest enzyme activity of 5452.633 unit at 72 hours fermentation, while isolate HL.45F.205 with 4725.58 units at 72 hours fermentation and isolate HL.110F.488 with 3167.609 units at 96 hours fermentation. Glucoamylase has been reproduced by isolate HL.44F.199 on volume media 100 ml. The results show that enzyme activity is 4197.10 units with specific activity 2851.44 U/mg proteins, both get an increasing result after filtration and ultra-filtration reach out 5910.86 units and 4534.45 U/mg proteins.

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Indonesia as an agricultural country rich in various types of plants including tubers. The purpose of this study was to produce and apply complex amylase enzymes from Aspergillus awamori KT-11 in hydrolyzing cassava and taro starch to liquid sugar. The production of complex amylase enzymes is carried out in a liquid and solid medium. The hydrolysis of starch into sugar is carried out with several enzyme concentrations (2-10%) and starch concentrations (5-25%) at room temperature (30ºC) and 60⁰C for 3 hours, 24 hours-72 hours. TLC tests were carried out on sugar from hydrolysis of cassava starch and taro flour to determine the type of sugar formed.As a result, the highest enzyme activity was obtained in the solid medium formation (242.83 Units/ml). The best condition for hydrolyzing starch is 15% starch concentration, 10% enzyme concentration at 60ºC for 72 hours. The results of hydrolysis sugar were 9.52 mg / ml (taro) and 9.37 mg/ml (cassava) respectively, with an incubation time of 72 hours.The TLC analysis showed that sugar Rf from cassava was detected as lactose and Rf from Talas flour as maltose.

Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant  (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.