Study on endophytic bacteria for bioactive compound production use as plant protection agent

Melliawati, Ruth; Widyaningrum, Dian Noverita; Djohan, Apridah Camelia; Sukiman, Harmastini

doi:10.13057/biodiv/d070305

Cited by 5 publications

(5 citation statements)

References 12 publications

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“…The variety of isolates obtained ranged from one to four isolates for each plant (Table 1). The results of this study are not much different from the results previously reported, ranging from one to six isolates per plant [13,14,15]. No endophytic bacteria were found in the plants of D. metel L. and S. cumini (L.) Skeels.…”

Section: Isolation and Purification Of Endophytic Bacteriacontrasting

confidence: 74%

“…F. oxysporum and S. rolfsii were from culture collection of Phytopathology Laboratory, Faculty of Agriculture, Andalas University, Padang, Indonesia. Screening of anti-phytopathogen activity was carried out disk diffusion method which refers to Melliawati et al (2006) [13]. Positive control used 15 ppm of chloramphenicol for bacteria, while positive control of fungus used 15 ppm of ketoconazole.…”

Section: Screening Of Endophytic Anti-phytopathogens Activitymentioning

confidence: 99%

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The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity

Zam

Agustien²,

Syamsuardi³

et al. 2019

JTLS

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Endophytic bacteria live in plant tissues which utilized in plant protection against phytopathogens. This study aims to investigate the diversity of endophytic bacteria from the leaves of traditional medicinal plants that has anti-phytopathogens properties. Isolation of endophytic bacteria was done by spread plate method. The bacteria were characterised by Gram staining and the 16S rRNA gene analysis. Further screening of anti-phytopathogen activity used disc diffusion method for Ralstonia solanacearum, Xanthomonas campestris, Fusarium oxysporum, and Sclerotium rolfsii. All togethers, sixteen isolates of endophytic bacteria from the leaves of eight medicinal plants species were obtained. Fourteen isolates had an anti-phytopathogen (with eight isolates against R. solanacearum, seven isolates against X. campestris, nine isolates against F. oxysporum, and five isolates against S. rolfsii). From the 14 isolates identified, phylum Firmicutes were dominant (64.3%), followed by Proteobacteria (28.6%), and Actinobacteria (7.1%). Phylum Firmicutes consists of Bacillus indicus (BJF1, TCF1, and MCF2), Bacillus pumilus (CAF4), Bacillus sp. (CAF1), Bacillus subtilis (AAF2, MCF1, CAF3, and MCF3); phylum Proteobacteria consists of Pantoea agglomerans (CAF2), Pantoea stewartii (AAF4), Pseudomonas oryzihabitans (AAF3), and Pseudomonas psychrotolerans (AAF1); and phylum Actinobacteria consists of Kocuria kristinae (CSF1).

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Section: Isolation and Purification Of Endophytic Bacteriacontrasting

confidence: 74%

Section: Screening Of Endophytic Anti-phytopathogens Activitymentioning

confidence: 99%

The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity

Zam

Agustien²,

Syamsuardi³

et al. 2019

JTLS

View full text Add to dashboard Cite

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“…Yuwatiningsih et al (2018) reported the possibility of amphotericin-B and voriconazole (antibiotics) from STG-1 isolates, which inhibits the growth of Fusarium oxysporum in the culture medium. Furthermore, the bioactive compoundproduced endophytes isolated from rare plants in the Indonesia National Park (Sumatra, Java, and Bali) and local plants in Yogyakarta effectively reduce pathogenic bacteria [6] and suppress the growth of fungal disease [7], respectively. In addition, antibacterial and cytotoxic compounds are also detected from bacterial endophytes isolated from Pahang National Park in Malaysia [8].…”

Section: Introductionmentioning

confidence: 99%

Potential of Karimunjawa Island’s Plants as Antibiotic-Producing Endophytic Bacteria Sources

Kumalasari

Kharisma

Yuwantiningsih

2022

Advances in Science and Technology

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Endophytic bacteria have a great potential to be applied as biofertilizers and biopesticides, but their information as a source of antibiotics still needs to be developed and explored. The aim of this study was to investigate the potential sources of antibiotics in endophytic bacteria isolated from the stems of Setigi, Wahong, Bongko, Kalimosodo, Dewandaru, and Legundi plants on Karimunjawa Island. Molecular approaches were performed to isolate, characterize, and identify bacterial endophytes as potential antibiotic sources by plate assay and 16S rRNA gene sequence analysis. Dewandaru isolate was identified as gram-negative bacteria, whereas; gram-positive bacteria were detected in other isolates. Moreover, Setigi and Dewandaru isolates showed the highest level to inhibit the growth of Fusarium sp and displayed 99% similarity with antibiotic-producing bacteria, namely Bacillus pumilus and Bacillus cereus, respectively. These results indicate the possibility of antibiotic activities by Setigi and Dewandaru isolated. Therefore, it is assumed that both Setigi and Dewandaru isolates potentially appeared as new antibiotics sources from local plants. This study provides novel insight into the future production of novel antibiotics derived from plant-associated endophytic bacterial as a strategy for increasing the application of natural compounds to control plant diseases in agriculture.

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“…It could protect plants from microbial pathogens attack such as Xanthomonas campestris, Pseudomonas solanacearum, Colletotricum gloeosporioides and Fusarium oxysporum, control of the disease in Siam citrusplants, banana plants (Melliawati et al, 2006;Hadiba, 2009;Munif and Hipi, 2011;Ningsih et al, 2012). Wibowo and Munif (2013) isolated endophytic bacteria from forest plants successfully such as from mahogany (Swietenia mahogany Jacq), tamarind (Albizia saman (Jacq.)…”

Section: Introductionmentioning

confidence: 99%

POPULATION DYNAMICS OF RHIZOBACTERIA AND ITS POTENCY AS A BIOLOGICAL CONTROL AGENT TO CONTROL FUSARIUM DISEASE IN THE NURSERY OF AGARWOOD (Aquailaria malaccensis Lamrk)

2015

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Agarwood is a resin product produced by particular trees and has a certain high comercial value. In Central Bangka Regency, agarwood is the main commodity of forest. The research was aimed to determine the dynamic population of rhizobacteria and its potential as a biological control agent to control Fusarium disease in the nursery of agarwood (Aquailaria malaccensis Lamrk). The research was carried out by using exploration and identification methods. Sixty nine bacterial isolates were obtained from 20 samples. The samples taken were from Pangkalan Baru and Koba districts. After selection process, 49 bacterial isolates were tested for the capacity of inhibition. Results showed that 37.50 % of the bacterial isolates indicated a strong inhibition capacity, meanwhile 58.33% indicated a moderate and only 4.70% possessed a weak inhibition. Pseudomonas fluorescens, P. aeroginosa, P. malthophilia and Klebsiella pnemoniae were identified from the selected isolates. These bacteria were potentially able to protect plants against Fusarium disease and to promote plant growth. This research needed to be continued at the field level in order to know the real effects on plant.

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Study on endophytic bacteria for bioactive compound production use as plant protection agent

Cited by 5 publications

References 12 publications

The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity

The Diversity of Endophytic Bacteria from the Traditional Medicinal Plants Leaves that Have Anti-Phytopathogens Activity

Potential of Karimunjawa Island’s Plants as Antibiotic-Producing Endophytic Bacteria Sources

POPULATION DYNAMICS OF RHIZOBACTERIA AND ITS POTENCY AS A BIOLOGICAL CONTROL AGENT TO CONTROL FUSARIUM DISEASE IN THE NURSERY OF AGARWOOD (Aquailaria malaccensis Lamrk)

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