Jokić S., Sudar R., Svilović S., Vidović S., Bilić M., Velić D., Jurković V. (2013): Fatty acid composition of oil obtained from soybeans by extraction with supercritical carbon dioxide. Czech J. Food Sci., 31: 116-125.

No abstract

Supercritical CO 2 extraction of soybean oil was performed. Response surface methodology was applied to evaluate the effects of pressure, temperature and extraction time on soybean oil yield. The analysis of variance showed that pressure and extraction time followed by the quadratic term of pressure and interaction between pressure and time had the significant effect on the oil yield. The maximum extraction yield (6.59 ⁄ 100 g soybeans) at constant CO 2 flow rate of 1.629 L per min was achieved at 50°C, 300 bar and 4 h. The experimental values agreed well with those predicted by regression model. One-stage diffusion model was successfully applied for modelling the kinetics of soybean oil. The main triacylglycerols of soybean oil were trilinolein, dilinoleoolein, dilinoleopalmitin and linoleooleopalmitin. Soybean oil extracted by supercritical CO 2 had higher levels of linoleic and linolenic acids and lower levels of palmitic and stearic acids compared to oil extracted by organic solvent.

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The usage and value of soybean (Glycine max (L.) Merr.) seed oil are mainly determined by its fatty acid composition, which affects physical, chemical and nutritional properties. In order to assess genotypes' suitability for edible oil production and determine the variability in phenotypic expression of the amount of oil and fatty acid composition in the seed, three-year (2010-2012) trials were set up with eight early maturing advanced soybean lines. As a result, we determined the amount of seed oil and composition of seed fatty acids (palmitic, stearic, oleic, linoleic and linolenic). After analysis of variance (ANOVA), we calculated the saturated fatty acid (SFA) amount, monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) ratio (MUFA:PUFA) as an indicator of the oxidative stability of the oil, and linoleic and linolenic acids ratio as an indicator of nutritional quality. To give further insight into divergence of given set of genotypes, cluster analysis was performed, while correlation analysis was used to better understand the relationships between all the traits investigated in this research, which play an important role in breeding procedures. The experimental and biometric results indicate the existence of variability in phenotypic expression of investigated traits with significant year and genotype effects, while cluster analysis divided genotypes into two main groups confirming the results of ANOVA. The amount of oil was higher than that obtained in earlier researches conducted in the same geographical region and all averages of five fatty acids coincided with the average values for commercial soybean varieties. MUFA:PUFA was lower than recommended for all tested genotypes, and linoleic and linolenic acids ratio values were higher than the recommended limits for vegetable oils. The results of the correlation study showed the significant and positive relationship between oil and oleic acid, while the significant and negative correlation between oil and both polyunsaturated fatty acids. According to the overall conclusions, the most suitable as a parental component in breeding programs aimed at creating genotypes for the production of edible oil was genotype OS-L-774, while genotypes OS-L-806 and OS-L-805 were the least suitable for the same purpose.

The crude protein and protein components of fourteen commercial bread wheat cultivars grown in Eastern Croatia over two years and three locations were evaluated. Protein components were measured by reversed phase -high pressure liquid chromatography. A dominant effect of genotypes was obtained for the proportion of albumins and globulins, γ-gliadins and total glutenins, while year had a more pronounced impact on crude protein, total gliadins and high molecular weights and low molecular weights glutenin subunits. Locations had the strongest influence on total extracted proteins. Variability of gliadins to glutenins ratio was at the same extent influenced by the genotype and the year. Among interactions, genotype x year and year x location had the highest impact on evaluated proteins. The principal component analysis showed that wheat cultivars were differentiated according to variability of grain protein components. Considering the significant impact of proteins on the wheat bread-making quality, cultivar classification by protein components significantly contributes to the improvement of breeding program towards the creation of high quality cultivars.

Fruit skin colour plays a major role in quality assessment of food, significantly determining consumer`s choice. Colour of sour cherries depends on anthocyanins which are phenolic compounds (flavonoids) present in high amounts in fruits. The aim of this study was to determine a possible relation between polyphenols (total phenolics and anthocyanins) and colour parameters of fruit skin of sour cherries. The plant material used in this study was twenty two sour cherry genotypes from an orchard of Agricultural Institute Osijek. Total phenolics and anthocyanins contents as well as colour parameters (L*, a*, b*, h and C) of fruit skin were determined. Variability between sour cherry genotypes in total phenolics and anthocyanins as well as in colour parameters was revealed. Total polyphenols content varied from 462.7 to 1049.0 mg GAE/100 g of fresh weight, while total anthocyanins ranged from 160.1 to 495.6 mg CGE/100 g of fresh weight. A significant positive correlation was found between polyphenols and anthocyanins content. Total phenolics content had a significant negative correlation with colour parameters b* and h, while anthocyanins content negatively correlated with colour parameters L*, b* and h. According to the obtained results, genotypes Maraska, Heimanns Konservenweichsel and Rexelle are the richest genotypes in polyphenols and anthocyanins content

-Drought is a limiting factor in fruit production today. Identification of sour cherry genotypes tolerant to drought will enable the sustainability of fruit production. The aim of our study was to select sour cherry genotypes according to their genetic background as well as drought tolerance and investigate possible mechanisms of drought tolerance through the changes in photosynthetic apparatus (i.e. photosynthetic pigment content) and photosynthesis process assessed through the chlorophyll fluorescence transient. All of them together with molecular markers (SSRs and AFLPs), relative water content (RWC) as indicator of plant water status distinguish two genotypes (Kelleris 16 and OS), which are the opposite in regards to drought tolerance. Down-regulation of photosynthesis in drought-treated Kelleris 16 plants was seen as changes in antenna complexes of PSII (decreased total chlorophylls content (a+b) and chlorophylls ratio (a/b)). Despite unchanged maximum quantum yield of PSII in drought-treated leaves of genotype OS, overall photosynthetic performance expressed as PI ABS was down-regulated in both investigated genotypes. However, decrement of PI ABS was much pronounced in genotype Kelleris 16, mainly because of changes in a certain fraction of RCs, which become dissipative centres, seen as increase in ABS/RC and DI 0 /RC, in order to avoid photooxidative damage of photosynthetic apparatus. Also, electron transport, seen as decrease in ET 0 /(TR 0 -ET 0 ) and ET 0 /RC, was impaired which lead to impaired CO 2 fixation and photosynthesis. The described changes in the functioning of photosynthetic apparatus in drought-treated plants of Kelleris 16 constitute the main distinction between the two investigated genotypes regarding drought adaptation mechanisms.