ABSTRAK Kulit buah Mundar (G. forbesii King.) merupakan tanaman endemik yang berasal dari Kalimantan Selatan yang berpotensi memiliki aktivitas antioksidan. Penelitian ini dilakukan untuk menguji aktivitas antioksidan ekstrak etanol 30% dan 70% kulit buah Mundar (G. forbesii King.) yang diperoleh dengan metode ekstraksi sokhletasi. Pengujian aktivitas antioksidan dilakukan dengan metode DPPH (2,2-Diphenyl-1-Picrylhydrazil) secara kualititatif dan kuantitatif dengan kuersetin sebagai kontrol positif. Hasil uji aktivitas antioksidan ekstrak etanol 30% dan 70% kulit buah Mundar (G. forbesii King.) secara kualitatif menunjukkan adanya aktivitas antioksidan yang ditandai dengan noda kuning pada plat kromatografi lapis tipis (KLT) setelah disemprot dengan larutan DPPH 0,1mM. Hasil uji aktivitas antioksidan secara kuantitatif menghasilkan nilai IC50 (Inhibitory Concentration) secara berturut-turut dari ekstrak etanol 30% kulit buah Mundar (G. forbesii King.); ekstrak etanol 70% kulit buah Mundar (G. forbesii King.); dan kuersetin adalah 717,01 ppm; 534,69 ppm; dan 2,04 ppm. Ekstrak etanol 70% kulit buah Mundar (G. forbesii King.) memiliki aktivitas antioksidan yang lebih baik dibandingkan ekstrak etanol 30% kulit buah Mundar (G. forbesii King.). Kata kunci: Kulit buah Mundar (G. forbesii King.), antioksidan, DPPH, kromatografi lapis tipis (KLT), IC50 ABSTRACT Antioxidants are compounds that can counteract free radicals. Mundar rind (Garcinia forbesii King.) is endemic plants from South Kalimantan that has the potential antioxidant activity. This research was conducted antioxidant activity test of ethanolic extract 30% and 70% of Mundar rind (G. forbesii King.) obtained by soxhlet extraction method. Antioxidant activity test of Mundar rind (G. forbesii King.) used the DPPH (2,2-Diphenyl-1-Picrylhydrazil) method qualitatively and quantitatively with quercetin as a positive control. The results of the antioxidant activity test qualitatively of ethanolic extract 30% and 70% of Mundar rind (G. forbesii King.) showed antioxidant activity marked with a yellow spot with purple background on kromatogram after sprayed with DPPH solution 0,1mM. The results of the antioxidant activity test quantitatively obtained IC50(Inhibitory Concentration) value respectively of ethanolic extract 30%; ethanolic extract 70%; and quercetin were 717,01 ppm; 534,69 ppm; and 2,04 ppm. The ethanolic extract 70% of Mundar rind (G. forbesii King.) had better antioxidant activity than the ethanolic extract 30% of Mundar rind (G. forbesii King.) Keywords: Mundar rind (G. Forbesii King.), antioxidant, DPPH, thin layer chromatography (TLC), IC50
Objective: The objective of this study was to evaluate the capability of fourier transform infrared (FTIR) spectroscopy in combination with multivariate calibration for prediction of free fatty acids (FFA) in Pangasius hypopthalmus (P. hypopthalmus) oil.Methods: FFA content in P. hypopthalmus oil was determined by attenuated total reflectance-FTIR spectroscopy. P. hypopthalmus oil derived from Pangasius’s meat (MP), and Pangasius’s liver and fat (LFP) were subjected to heat treatments. Determination of FFA content in P. hypopthalmus oil’s was performed by gas chromatography-flame ionization detector.Results: Oleic acid was found to be the main fatty acid component in P. hypopthalmus oil. FTIR spectra of P. hypopthalmus oil has 3 main peaks, C-H bonds of cis-form of fatty acid showed the stretching vibration, symmetric and asymmetric vibrations of the C-H2 and C-H3 aliphatic group and vibrations of the carbonyl (C=O) ester derived from the oil triacylglycerols. Principal component regression (PCR) model showed a better performance than the partial least square (PLS) model. PCR at wavenumbers of 1200-1000 cm-1 with first derivative treatment was chosen for FFA prediction, which resulted in a coefficient of determination (R2) value of 0.9417, root means square error of calibration (RMSEC) of 0.725%, and root mean square error of prediction (RMSEP) value of 2.40%, respectively.Conclusion: FTIR spectroscopy combined with PCR can be used as an alternative method for analysis of fatty acid contents.
Tandui (Mangifera rufocostata Kosterm.) is a typical plant from South Kalimantan which belongs to the genus of Mangifera. Several species of Mangifera are known to have antioxidant activity. This study aimed to determine the antioxidant activity of ethanol extract of Tandui leaves. Tandui leaves that were obtained from the maceration method used 70% ethanol. Antioxidant activity was conducted quantitative using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. The result of the antioxidant activity of Ethanol extract of Tandui leaves quantitatively obtained IC50 value was 60.7042 �g/mL. The ethanol extract of Tandui leaves has strong antioxidant activity
Kelompok Wanita Tani (KWT) Cantik Manis merupakan salah satu Kelompok Wanita Tani (KWT) aktif yang berada di bawah naungan UPT BPP Liang Anggang Banjarbaru yang berada di Kelurahan Landasan Ulin Barat. KWT Cantik Manis saat ini tengah aktif membudidayakan tanaman hias Bayam Brazil, yang saat ini hanya digunakan untuk konsumsi sebagai campuran olahan sayuran, keripik dan tanaman hias dipekarangan rumah. Melihat potensi Bayam Brazil, STIKES Borneo Lestari Banjarbaru melalui program pengabdian masyarakatnya membantu memberikan edukasi melalui pengembangan pemanfaatan Bayam Brazil menjadi produk yang lebih bernilai ekonomis seperti nugget dan sirup. Kegiatan pengabdian ini bertujuan untuk mengenalkan masyarakat tentang cara pengolahan dan pemanfaatan Bayam Brazil. Kegiatan dilakukan dengan memberikan materi (edukasi) tentang antioksidan alami, khasiat dari Bayam Brazil dan demo atau praktek pembuatan sirup dan nugget dari bahan Bayam Brazil. Berdasarkan hasil analisis data terlihat antusias masyarakat terkait kegiatan tersebut. Hal ini karena produk olahan nugget dan sirup ini dapat menjadi salah satu alternatif dalam menjalankan wirausaha produk yang berpotensi berkembang di masyarakat untuk mendukung terwujudnya masyarakat tangguh di era new normal dalam usaha peningkatan derajat ekonomi masyarakat. Masyarakat juga mendapatkan wawasan baru tentang antioksidan alami, khasiat Bayam Brazil dan demontrasi pengolahan nugget dan sirup.
ABSTRAKAntioksidan memiliki peranan penting dalam menghambat radikal bebas pemicu terjadinya stress oksidatif sel penyebab berbagai penyakit degeneratif. Kalangkala (Litsea angulata) merupakan salah satu tumbuhan khas Kalimantan yang diduga berpotensi sebagai antioksidan alami pencegah stress oksidatif. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari ekstrak etanol daun dan kulit batang kalangkala (L.angulata) asal Kalimantan Selatan. Pengujian aktivitas antioksidan dilakukan secara kualitatif dan kuantitatif menggunakan metode DPPH. Secara kualitatif, hasil uji aktivitas antioksidan ekstrak etanol daun dan kulit batang kalangkala (L.angulata) menunjukkan adanya bercak kuning berlatar ungu pada plat KLT. Hasil kuantitatif ekstrak etanol daun kalangkala (L.angulata) diperoleh nilai IC 50 sebesar 152,39 ppm, sedangkan ekstrak etanol kulit batang kalangkala (L.angulata) diperoleh nilai IC 50 sebesar 85,33 ppm. Berdasarkan hal tersebut maka dapat disimpulkan bahwa daun dan kulit batang tumbuhan kalangkala (L.angulata) memiliki aktivitas antioksidan.Kata kunci: Kalangkala, Litsea angulata, daun, kulit batang, antioksidan ABSTRACT Antioxidants have an important role in inhibiting free radicals that trigger oxidative stress cells that cause degenerative diseases. Kalangkala (Litsea angulata) is a typical plant of Borneo which may have antioxidant activity. This study aims to determine the antioxidant activity of ethanol extract of leaves and stem bark of Kalangkala (L.angulata) from South Borneo. Extracts were investigated for antioxidant activity qualitatively and quantitatively using 2,2diphenyl-1-picrylhydrazyl (DPPH). Qualitatively, the results of antioxidant assays from ethanol extract of leaves and stem bark showed that there were yellow spots with a purple background on the TLC plate. Quantitatively, the ethanol extract of Kalangkala leaves obtained IC 50 values of 152.39 ppm, while the ethanol extract of Kalangkala stem bark obtained IC 50 values of 85.33 ppm. Thus, it can be concluded that the leaves and stem bark of Kalangkala (L.angulata) have antioxidant activity.
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