Infrared Spectroscopy and Multivariate Calibration for the Rapid Quantification of Free Fatty Acid Content in Pangasius Hypopthalmus Oil

Abstract

Objective: The objective of this study was to evaluate the capability of fourier transform infrared (FTIR) spectroscopy in combination with multivariate calibration for prediction of free fatty acids (FFA) in Pangasius hypopthalmus (P. hypopthalmus) oil.Methods: FFA content in P. hypopthalmus oil was determined by attenuated total reflectance-FTIR spectroscopy. P. hypopthalmus oil derived from Pangasius’s meat (MP), and Pangasius’s liver and fat (LFP) were subjected to heat treatments. Determination of FFA content in P. hypopthalmus oil’s was performed by gas chromatography-flame ionization detector.Results: Oleic acid was found to be the main fatty acid component in P. hypopthalmus oil. FTIR spectra of P. hypopthalmus oil has 3 main peaks, C-H bonds of cis-form of fatty acid showed the stretching vibration, symmetric and asymmetric vibrations of the C-H2 and C-H3 aliphatic group and vibrations of the carbonyl (C=O) ester derived from the oil triacylglycerols. Principal component regression (PCR) model showed a better performance than the partial least square (PLS) model. PCR at wavenumbers of 1200-1000 cm-1 with first derivative treatment was chosen for FFA prediction, which resulted in a coefficient of determination (R2) value of 0.9417, root means square error of calibration (RMSEC) of 0.725%, and root mean square error of prediction (RMSEP) value of 2.40%, respectively.Conclusion: FTIR spectroscopy combined with PCR can be used as an alternative method for analysis of fatty acid contents.

Analysis of Adulterated Pangasius Hypopthalmus Oil by ATR-FTIR Spectroscopy and Chemometric

“…This shows that the composition of the MP, LFP, CO and PO is almost the same. 3) and ( 4) with sharp peaks and adjacent around 2954 and 2852 cm -1 indicate the stretching C-H vibrations of carbon aliphatic group in methyl and methylene [3,23]. Band (5) at 1742-1744 cm -1 is specifically showed the stretching vibration of C=O [3,22].…”

“…3) and ( 4) with sharp peaks and adjacent around 2954 and 2852 cm -1 indicate the stretching C-H vibrations of carbon aliphatic group in methyl and methylene [3,23]. Band (5) at 1742-1744 cm -1 is specifically showed the stretching vibration of C=O [3,22]. Spectral patterns in fingerprint region at 1400-720 cm -1 are identical, this makes it difficult to distinguish between the four types of oil by only using the FTIR spectrum.…”

“…Pangasius hypopthalmus (P. hypopthalmus) oil contains polyunsaturated fatty acids (PUFA) which can be beneficial to human health [1,2]. The high production of catfish in Indonesia provides a great opportunity for the production of P. hypopthalmus oil [3]. In edible oil trade, oil adulteration has become a global issue.…”

The Authentication of Virgin Coconut Oil From Grape Seed Oil and Soybean Oil Using Ftir Spectroscopy and Chemometrics

“…Valid and reliable analytical methods have been reported for the authentication of VCO, namely fast gas chromatography [8], differential scanning calorimetry [9], electronic nose [10], nuclear magnetic resonance phosphorus-31 (31 P FTIR spectroscopy combined with some chemometrics techniques of multivariate calibration of partial least square and discriminant analysis have been successfully used for authentication of VCO from some lower-priced fats and oils, namely lard from palm kernel oil [13], VCO from palm oil [14], corn and sunflower oils [15]. FTIR spectroscopy in combination with chemometrics was also used for authentication of candlenut [16] and for analysis of fatty acids [17]. However, the application of FTIR spectroscopic method for authentication of soybean oil and grape seed oil having a different characteristic with those oils has not been reported yet.…”