No abstract

No abstract

No abstract

In this article, we describe the synthesis and biological evaluation of a novel 2-(methylamino)-3-nitro-4-(4-oxo-4Hchromen-3-yl) pyrano[3,2-c]chromen-5(4H)-one (CCN). It is also determined that CCN impregnated into the collagen scaffold has the potential to mimic the function of the extracellular matrix as a biomaterial in the field of tissue engineering. The series of pyrano[3,2-c]chromen-5(4H)-one derivatives (4a-4j), were analyzed by 1 H NMR, 13 C NMR, Mass spectral and FTIR analysis. The compound 4c confirmed by single crystal XRD studies. All the compounds were screened for antimicrobial activity against the gram positive, gram negative bacteria and yeast. Among all the compounds, the compound (4a=CCN) showed activity against Gram positive and Gram negative bacteria, when compared to the synthesized compounds. Further the compound CCN was evaluated for cytotoxicity against MCF-7, Hep-2 and Vero cancer cell lines with IC50 values of 5.4 µg/ml, 5.3 µg/ml and 68.4 µg/ml respectively. In addition, the result of flow cytometry and docking ((PDBID: 1A27 with the ligand) study were supported to the activity of the synthesized compound (4a). The FTIR and NMR analysis of the CCN impregnated collagen scaffold were done to reveal the existence of CCN molecule in the scaffold. The inherent property of the collagen scaffold was not significantly affected by structure of CCN molecule. The thermal and mechanical properties of the collagen scaffold impregnated with CCN molecule gives stability as well as supports the swelling. However, the COL-CCN scaffold showed an enhanced cell attachment and proliferation of NIH 3T3 fibroblast cells. Based on the results, the novel CCN molecule impregnated with collagen scaffold has the potential application as a biomaterial in tissue engineering.

The original version of this article unfortunately contained a mistake in acknowledgement section. The corrected acknowledgement is published with this erratum article.

A facile atom economic and eco friendly protocol for the synthesis of biologically important quaternary centered C-3 functionalized oxindole derivatives, with a novel nucleus, in high yields has been demonstrated.

Since ancient times, medicinal plants have been widely used against dreadful pathogens due to their ability to kill microbes. Also, the limitations of the present treatment methods have led the researchers to explore for new formulations with aspects of better wound healing and control of infections. In the present study antimicrobial, antioxidant and cytotoxicity properties of a polyherbal formulation prepared from the barks of three medicinal plants for treating wound infection were investigated. The polyherbal formulation was tested for broad spectrum antimicrobial activity against gram-positive bacteria Methicillin-Resistant Staphylococcus aureus (MRSA) and gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and antifungal activity against Fluconazole resistant Candida albicans. The result reveals that the polyherbal formulation exhibited antimicrobial activity against antibiotic resistant gram-positive MRSA and C. albicans and moderate against gram-negative P. aeruginosa, E. coli and S. typhi. The antioxidant activities like 1,1′-diphenyl-2-picrylhydrazyl and hydrogen peroxide scavenging ability of the formulation was compared with standard ascorbic acid. The higher antioxidant activity was found mostly due to the presence of higher amount of phenols and flavonoids. The phenol was found to be 48.63 ± 0.35 mg/g and flavonoids was 29.52 ± 1.13 mg/g. The polyherbal formulation helps in the proliferation of fibroblast cells without affecting the viability. The above result reveals that the formulation has good antimicrobial and antioxidant activity without affecting the viability of the cell.

Magnesium oxide nanoparticles (MgONPs) synthesized by efficient green approach have unique physiochemical properties. In this study, MgONPs are synthesized with bract extract of Musa acuminate, an agro waste. The surface plasmon resonance at 450 nm in UV spectrum and FTIR peaks at 601 and 890 cm−1 confirmed the presence of MgONPs. XRD pattern revealed high crystallinity of the nanoparticles with an intense orientation peak at 111, and the size was 13 nm. The particles were spherical with an average size of 24.85 nm. The elemental percentage of magnesium and oxygen were 68.55% and 31.45%. MgONPs had antibacterial activity against Bacillus subtilis, Escherichia coli, Vibrio harveyi, Vibrio parahemolyticus, and Staphylococcus aureus with MIC, 6 μg/mL. The IC50 value for MCF‐7 cell was 113.56 μg/mL, and the normal cell line was 785.69 μg/mL. The NPs also exhibited hemolytic features in a dose‐dependent manner. The MgONPs exhibited photocatalytic degradation of methyl violet, CBB G‐250, and malachite green in 60 min duration. MgONPs had promising antibacterial, cytotoxic, hemolytic, photocatalytic, and seed germination activity. They have the potential to serve as an additive in a variety of biological applications.