The importance and use of herbal remedies (turmeric, tulsi, amla and aloe vera etc.) has been reviewed systematically. Turmeric (curcumin), acts as an antioxidant, antimutagenic, antiinflamatory and antimicrobial agent and protect liver against a variety of toxicants. Tulsi (eugenol) have anticancer properties, reduced blood glucose levels, total cholesterol levels and promotes immune system function. Amla, richest source of vitamin-c and it's active tannoid principles have antimicrobial, antidiabetics, anticarcinogenic properties and enhances immune property. Aloe vera contains phytochemicals (Saponins, flavonoids, alkaloids and phenols), which is an indication of cosmetic and medicinal value. Turmeric, tulsi, amla and aloe vera preparations increased the body weight gain, feed efficiency and decreased the feed intake. These preparations decreased the mortality rates and the cost of feed has been decreased from 6.2% to 13.5%. They have reduced the fat accumulation, increased dressing percentage, liver weight, spleen weight and whole giblet weights. Significant reduction of serum cholesterol, serum triglycerides and increased the humoral response against RD vaccine.

Introduction: Salmonella enterica subspecies enterica serovar Typhimurium ( S. Typhimurium ) is one of the major cause of foodborne zoonoses in humans. Poultry acts as a reservoir for S . Typhimurium without showing clinical signs and has become a source of infection to humans. Besides, it also became a source of multidrug-resistant (MDR) strains of S . Typhimurium. Methods: In the present study, we have isolated 9 S . Typhimurium from 503 samples from environmental sources of poultry wet markets in the Kadapa District of Andhra Pradesh, India. The role of efflux pump activity in antibiotic resistance was evaluated by ethidium bromide cartwheel test and efflux pump inhibition assay. Results: Eight out of nine isolates were resistant to two or more classes of antibiotics. The efflux pump activity of these isolates by ethidium bromide cartwheel method revealed that 66.6% isolates had shown evidence of pronounced efflux activity. The zone of inhibition (ZOI) of resistant antibiotics for each isolate was estimated in the presence or absence of 25 µg/mL of PAβN. Overall, cephalosporins (cefazolin, cefamandole, and cefaclor), kanamycin, polymyxin-B, piperacillin, and imipenem showed significant increase (≥2 mm) of ZOI, indicating the role of efflux pumps for efflux of these drugs. A maximum of 4 antibiotics among EtBr efflux pump positive isolates and 2 antibiotics among EtBr efflux pump negative isolates showed increased ZOI in the presence of PAβN. Conclusion: The results indicate that efflux pumps of MDR S . Typhimurium may contribute to resistance for at least one antibiotic, even in EtBr cartwheel test negative isolates.

Aim: To test the sensitivity of S. Enteritidis for selected antibiotics. Materials and Methods: S. Enteritidis isolates obtained from different samples of chicken, mutton, turkey meat, faecal and cloacal samples of poultry and turkey, eggs, water and feed were subjected for sensitivity and resistance to selected antibiotics like- Chloramphenicol (30μg), Gentamicin (10 μg), Nalidixic Acid (30 μg), Tetracycline (30 μg), Ciprofloxacin (5 μg), Amikacin (30 μg), Amoxicillin (25 μg), Ampicillin (10 μg), Streptomycin (10 μg) and Sulfonamide (30 μg). Antimicrobial susceptibility of the isolates was established by the disk diffusion assay with MH (Muller-Hinton) agar in accordance with French National Antibiogram Committee Guidelines. Results: The sensitivity of S. Enteritidis was 100% for ciprofloxacin followed by chloramphenicol and amikacin (96%), gentamycin (90%), amoxicillin (82%), streptomycin (80%), tetracycline (76%), nalidixic acid (68%), ampicillin (58%) and sulfonamide (10%). The resistance was highest for sulfonamide (76%) followed by ampicillin (32%), nalidixic acid (30%) and 6-20% for gentamycin, amoxicillin and tetracycline. Conclusion: S. Enteritidis isolates were more sensitive to ciprofloxacin, chloramphenicol, amikacin, gentamycin, streptomycin,amoxicillin and tetracyclines and less sensitive to sulfonamides. Higher resistance was observed with sulfonamide followed by ampicillin and nalidixic acid. [Vet World 2013; 6(4.000): 185-188

Shiga toxin-producing Escherichia coli (STEC) strains are considered the most common food-borne zoonotic pathogen and are highly pathogenic to humans in low infectious doses, causing food-borne diseases through consumption of contaminated water or food. Resistance against antibiotics by STEC is a big concern nowadays. Two hundred farm water samples were collected aseptically from different livestock farms in and around Proddatur, Andhra Pradesh, India and Processed for E. coli isolation, identification with culture method and molecular characterization by PCR. E. coli was characterized as STEC with two genes i.e. stx1 and stx2. All the STEC isolates were subjected to an antibiotic sensitivity test by disc diffusion method against ten antibiotics. Results showed that out of 200 farm water samples, 196 were positive for E. coli with an overall prevalence of 98% (196/200) and 62.2% (122/196) for STEC by PCR. Antimicrobial susceptibility test by disc diffusion method against ten antibiotics revealed the higher resistance to Cepahlothin (100%) followed by Tetracyclin (98.4%), Ampicillin (96.7%), Streptomycin (95%), Sulphonamides (91.8%), Trimethoprim (84.4%), Kanamycin (34.4%), Chloromphenicol (17.2%), Colistin (9.0%) and least resistance to Gentamycin (4.9%).

No abstract