Molecular Characterisation and Antimicrobial Resistance Patterns of Shiga Toxin Producing Escherichia coli Isolated from Farm Water Samples

Abstract

Shiga toxin-producing Escherichia coli (STEC) strains are considered the most common food-borne zoonotic pathogen and are highly pathogenic to humans in low infectious doses, causing food-borne diseases through consumption of contaminated water or food. Resistance against antibiotics by STEC is a big concern nowadays. Two hundred farm water samples were collected aseptically from different livestock farms in and around Proddatur, Andhra Pradesh, India and Processed for E. coli isolation, identification with culture method and molecular characterization by PCR. E. coli was characterized as STEC with two genes i.e. stx1 and stx2. All the STEC isolates were subjected to an antibiotic sensitivity test by disc diffusion method against ten antibiotics. Results showed that out of 200 farm water samples, 196 were positive for E. coli with an overall prevalence of 98% (196/200) and 62.2% (122/196) for STEC by PCR. Antimicrobial susceptibility test by disc diffusion method against ten antibiotics revealed the higher resistance to Cepahlothin (100%) followed by Tetracyclin (98.4%), Ampicillin (96.7%), Streptomycin (95%), Sulphonamides (91.8%), Trimethoprim (84.4%), Kanamycin (34.4%), Chloromphenicol (17.2%), Colistin (9.0%) and least resistance to Gentamycin (4.9%).

Effects of mixed fibres and essential oils blend on growth performance and intestinal barrier function of piglets challenged with enterotoxigenic Escherichia coli K88

“…To our knowledge, diarrhoea is one of the most serious diseases causing mortality and morbidity in piglets, and leads to serious economic losses in swine industry (Zhang et al, 2018). Currently, we used ETEC, the master causative agent for diarrhoea (Tumlam et al, 2022), to establish a diarrhoea challenge model in weaned pigs. Compared to CON group, ETEC challenge significantly increased the diarrhoea incidence, however, which was alleviated by AT, MDF, EOB and MDF + EOB.…”