Tea leaves have economic importance in preparation of the popular beverage of the world "tea". Bird's eye spot disease of tea leaves creates significant revenue loss in tea trade of many tea plant cultivating countries. Management of this disease by silver (AgNps) and copper (CuNps) nanoparticles that are biosynthesised by efficient antagonists was studied. The biocontrol agents like Pseudomonas fluorescens, Trichoderma atroviride and Streptomyces sannanensis were evaluated for nanoparticle synthesis against Cercospora theae isolates namely KC10, MC24 and VC38. Initially, the freshly prepared extracellular AgNps showed high disease control (59.42 - 79.76%), but the stability of antagonistic property in stored nanoparticles were significantly high in CuNps (58.71 - 73.81%). Greenhouse studies on various treatments imposed also showed reduced disease incidence percentage of 13.4, 7.57 and 10.11% when treated with CuNps synthesized by P. fluorescens, T. atroviride and S. sannanensis respectively. Various treatment schedule in fields suggested the use of Bionanocopper@1.5 ppm for highest yield (3743 kg/ha) with 66.1% disease prevention. The results suggest the use of biosynthesised CuNps using Streptomyces sannanensis for controlling the tea plant pathogens causing foliar disease with higher stability in releasing the antagonistic activity during sporadic disease incidence of bird's eye spot disease in tea plants.
Receptor tyrosine kinase is a large transmembrane protein family that is often deregulated to cause lung cancer. EGFR is a cell membrane receptor that is over-expressed in lung cancer with numerous active mutated genes. Pharmacologically active compounds were purified from methanolic leaf extract of Schefflera stellata (Geartn.) Baill. which shows the potent inhibitory effect on over-expressed EGFRs. FTIR analysis D4 and D5 shows the presence of different functional groups such as carboxylic acids, phenol, amines, alcohols, alkanes, alkenes, aromatics, alkyl halides, esters, aromatic amines, ethers, aliphatic amines, saturated aliphatic compounds with 14 major characteristics peaks of bioactive components. Docking studies were carried out between the EGFR tyrosine kinase and purified bioactive compounds (D4 and D5). Totally 5 bioactive molecules were docked with both wild and mutated EGFR. The 3 ligand molecules were picked out based on their binding energy. Among 3 bioactive molecules 1, 2-benzene dicarboxylic acid, mono (2-Ethylhexyl) ester (CID 20393) was found to be most effective that inhibiting over-expressed EGFR tyrosine kinase. The results confirmed that the purified bioactive compounds of Schefflera stellata (Geartn.) Baill. methanolic leaf extract possessed different bioactive functional constituents and its inhibitory role in binding with the D5 compound in over-expressed EGFR in lung cancer cells using docking studies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.