Obesitas merupakan kelompok penyakit tidak menular yang menjadi kesehatan dunia dan muncul sebagai epidemi baik di negara maju ataupun berkembang. Konsumsi gula yang berlebihan diketahui merupakan faktor risiko obesitas dan dapat berujung pada penyakit Diabetes Mellitus Type 2 (T2DM)dengan angka mortalitas yang tinggi. Salah satu upaya untuk mengurangi kebiasaan mengkonsumsi gula berlebih dalam upaya mencegah obesitas sesuai anjuran pemerintah adalah penyuluhan tentang pentingnya pembatasan konsumsi gula bagi kesehatan. Upaya ini juga telah dilakukan melalui Program Pembangunan Kesehatan Masyarakat, PPKM) kepada masyarakat Desa Sumberlerak, Kabupaten Boyolali, Jawa Tengah, Indonesia, menggunakan poster sebagai media sosialisasi. Tujuannya adalah untuk meningkatkan masyarakat di desa tersebut terkait hubungan konsumsi gula berlebih dengan risiko dan gejala klinis dari obesitas dan DM. Pendekatan partisipatif berupa penyuluhan dengan media poster buatan mahasiswa telah dilakukan diikuti evaluasi berupa pengisian kuesioner sebelum dan sesudah penyuluhan. Berdasarkan hasil analisis kuesioner yang telah dilakukan, PPKM berupa penyuluhan menggunakan poster sebagai media komunikasi dapat meningkatkan kesadaran tentang bahaya konsumsi gula berlebih pada masyarakat Desa Sumberlerak, Kabupaten Boyolali Jawa Tengah.
Hydrolytic bacteria are known for their crucial role in the bioconversion of organic matters mainly present in biomedical waste, making them advantageous in bioremediation. Analysis of abundance and colony diversity of culturable bacteria producing hydrolytic enzymes isolated from liquid biomedical waste of two different classes of hospitals in Semarang city, Central Java was carried out. Bacterial cultivation on nutrient agar slants resulted in total 26 viable colonies from inlet samples of Rumah Sakit Umum Roemani Muhammadiyah (R1) and Rumah Sakit Umum Daerah KRMT Wongsonegoro (R2) hospitals. Both hospitals represented two different classes of hospitals in Semarang (C and B, where B class is higher grade). Screening for production of four main hydrolytic enzymes (protease, lipase, amylase and cellulose) on all cultivable colonies obtained in this study. The results showed that all of them were capable of producing more than one type of hydrolytic enzymes. The composition was as follows: Protease producers (26 colonies), amylase producers (14 colonies), cellulose producers (13 colonies) and lipase producers (10 colonies).This study demonstrated that primary inlet of hospitals is a rich source of hydrolytic bacteria, where extracellular protease producers were found to be the main population of this group. In Semarang case, the inlet of lower class of hospital appeared to be more potential source of these hydrolytic bacteria than that of higher class one.
Introduction: Injection of intraperitoneal gentamicin at a dose of 60 mg/kg BW intra-peritoneal in rats for 7 days has been shown to trigger renal tubular degeneration and increase in high sensitivity C-reactive protein (hs-CRP) level. The use of purslane (Portulaca oleracea) to reduce hs-CRP levels and total score of renal tubular degeneration has not been reported.Objective: to determine the effect of purslane ethanol extract on hs-CRP levels and renal tubular degeneration score in gentamicin induced-renal damage in rats.Methods: This was a study using posttest only control group design. Twentyfive male Wistar rats aged 8-12 weeks, weighed 150-200 grams were allocated into 5 groups: normal (Nor-G), without treatment; negative (Neg-G), gentamicin (60 mg/kg BW), intraperitoneally; P-200; P-300 ; P-400 (gentamicin plus purslane extract at a dose of 200, 300, and 400 respectively) for 7 days. The hs-CRP was evaluated using the ELISA method. Total score of renal tubular degeneration was evaluated using modified score of Sarjadi.Results: there was a significant difference in mean hsCRP level and total score of renal tubular degeneration between groups (p <0.05). Posthoc analysis showed that hsCRP level and total score group of P-200, P-300 and P-400 were significantly lower than those of Neg-G, p <0.05. Meanwhile, the levels of hsCRP and total score of renal tubular degeneration in P-200, P-300, P-400, and Nor-G groups were not significantly different (p> 0.05).Conclusion: the administration of purslane ethanol extract at doses of 200, 300, and 400 mg/kg BW for 7 days improve hs-CRP level and total score of tubular degeneration similar to normal.
Demam berdarah (DB) / dengue fever (DF) adalah penyakit yang ditularkan melalui gigitan nyamuk, terutama Aedes aegypty atau Aedes albopictus sebagai vektor dari virus dengue. Penyebab dari DF adalah infeksi dari salah satu virus dari 4 serotipe virus dengue (DENV-1, 2, 3, dan 4). Pengendalian vektor virus dengue dapat dilakukan dengan gerakan pemberantasan sarang nyamuk (PSN) 3M plus. Tujuan kegiatan pengabdian masyarakat pada kelompok PKK RT. 6 RW.28 Bukit Mutiara Jaya III adalah untuk meningkatkan ketentraman masyarakat melalui peningkatan kesehatan dan penerapan aplikasi BUTIK untuk mempermudah PSN dan mempercepat proses pelaporan. Metode pelaksanaan pengabdian masyarakat dilakukan dengan survey lokasi, mengurus permohonan menjadi mitra dan ijin kegiatan kepada ketua PKK. Pelaksaan pengabdian kepada masyarakat meliputi sosialisasi penggunaan aplikasi BUTIK, anggota PKK memasang aplikasi pada smartphone milik pribadi, kemudian pelaksanaan PSN ke rumah warga dengan menggunakan aplikasi BUTIK. Hasil kegiatan berdasarkan kuisioner menjunjukkan 97% peserta setuju bahwa aplikasi BUTIK membantu dan bermanfaat dalam pelaksanaan PSN. Kesimpulan dari kegiatan pengabdian masyarakat yang telah dilakukan adalah Aplikasi BUTIK telah disosialisasikan dan diterapkan kepada kelompok PKK Rt. 6 Bukit Mutiara Jaya III dan peserta merasa aplikasi BUTIK bermanfaat untuk peningkatan kesehatan dan mempermudah pelaporan PSN di kelompok PKK Rt. 6 Bukit Mutiara Jaya III.
Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.
Antibiotics from the aminoglycoside group, such as gentamicin, are frequently used for the infection therapy of gram-negative bacteria including Salmonella typhi. Some studies show that gentamicin can cause hepatotoxicity and increase the level of SGPT (serum glutamic pyruvic transaminase). The purpose of this research is to figure out the decreasing SGPT level of male Wistar rats induced by gentamicin with the purslane ethanol extract. This research employed a post-test only control group design, utilizing 25 male Wistar rats divided into 5 groups. The normal control group (NorG) was without any treatment, while the positive control (PG) was intraperitoneally injected with gentamicin 60 mg/kg of rat body weight. The treatment groups consisted of P1, P2, and P3 were intraperitoneally injected with gentamicin at the dosage of 60 mg/kg of rat body weight for 7 days and then administered with purslane ethanol extract respectively at the dosage of 200, 300, and 400 mg/kg of rat body weight per oral for 7 days. The analysis on the SGPT level was conducted with the IFCC modification method using chemistry analyzer. One way ANOVA test shows that there were significant differences in SGPT levels among groups. LSD post hoc test shows that purslane ethanol extract at the dosage of 400 mg/kg of rat body weight significantly decreased the SGPT level (p<0.05) when compared to the positive control group. The administration of common purslane ethanol extract at the dosage of 200, 300, and 400 mg/kg of rat body weight can decrease the SGPT level of male Wistar rats induced by gentamicin.
Type 2 diabetes mellitus is a metabolic disorder characterized by hyperglycemia as a result of reducing insulin secretion of pancreas beta cells or insulin function disorder. Type 2 DM can cause lipid metabolism disorder (dyslipidemia) such as increasing total cholesterol, triglyceride, and decreasing HDL levels. The small and dense , Rafparticle of LDL is susceptible to oxidation, so it makes LDL more atherogenic than other lipoproteins. A high level of LDL is a factor in atherosclerosis. It can be affected by patients’ lifestyles. The aim of this study is to figure out the relation between fasting blood sugar and LDL Cholesterol of Type 2 DM Patients on William Booth Hospital Semarang. This study used secondary data taken from 85 patients from the medical report and laboratory test results of William Booth Hospital Semarang from January to May 2021 that fit inclusion and exclusion criteria. Result data were analyzed by a statistical test with Spearman’s correlation test. The result of this study showed that 96,5% of patients had high levels of fasting blood sugar and 76,4% of high levels of LDL cholesterol, with Spearman's correlation p-value was 0,977. The study concluded that there is no meaningful correlation between fasting blood sugar and LDL cholesterol of type 2 DM patients on William Booth Hospital Semarang.
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