During the late summer of 2016, a pattern of infection spots were observed in different varieties of Prunus armeniaca (Apricot) and Prunus persica (Peaches) in different locations in India. The disease led to a decline in the fruit production by almost 30% in the region. Symptomatologically, the dark brown leaf spots were hologenous, scattered over the entire surface, rounded to irregular, enlarging and coalescing, which resulted in premature defoliation. Both phenotypic & genotypic observations were carried out by double beam Scanning Electron Microscope (SEM) and Internal Transcribed Spacer (ITS) rDNA sequencing, respectively. To our knowledge it is the first report of this disease from India and solid containment measures should be imposed to restrict its spread throughout the natural range of these ecologically, economically and historically precious hosts.
During the summer 2014, dark black symptoms beneath the leaf surfaces were observed on the Populas alba L species in the south Kashmir. In the later stages of development the infection subtends the whole surface resulting in leaf collapse. Fungal isolates were recovered directly from the structures present on the lesions. Purified DNA from each isolate was amplified using the random amplified polymorphic DNA technique recruiting the ITSI (TCCGTAGGTGAACCTGCGG) and ITS4 (TCCTCCGC TTATTGATATGC) specific primers. Amplification products visualized on agarose gel showed specific band pattern for each isolate. The mycotaxonomic characterization and phylogenetic interpretations showed the emergence of fungal species, Meliola sp. KY623717 with genetic variance of 2% in internal transcribed spacer (ITS) region of rDNA from closely related species under geographically distinct region.
During the summer 2014, dark black symptoms beneath the leaf surfaces were observed on the Populas alba L species in the south Kashmir. In the later stages of development the infection subtends the whole surface resulting in leaf collapse. Fungal isolates were recovered directly from the structures present on the lesions. Purified DNA from each isolate was amplified using the random amplified polymorphic DNA technique recruiting the ITSI (TCCGTAGGTGAACCTGCGG) and ITS4 (TCCTCCGC TTATTGATATGC) specific primers. Amplification products visualized on agarose gel showed specific band pattern for each isolate. The mycotaxonomic characterization and phylogenetic interpretations showed the emergence of fungal species, Meliola sp. KY623717 with genetic variance of 2% in Internal Transcribed Spacer (ITS) region of rDNA from closely related species under geographically distinct region.
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