In mid-summer 2015, during the mycological explorations to the northern India (Kashmir), canker stain symptoms in alarming proportions were observed on beneath of the leaf surfaces in 21 differentially located Platanus orientalis kashmeriana populations similar to what has been reported earlier in United States and Europe. Genetic interpretations of these isolates, using ITS region of rDNA as unique DNA barcoding site for molecular identification showed the evolution of new fungal species, Alternaria kashmeriana sp.nov. From closely related species under geographically distinct conditions encountering 0.5 amount of genetic change in ITS region of rDNA. It is hard to differentiate the Alternaria Kashmeriana sp.nov and from the rest known Alternaria species on the phenotypic bases but reflects the marked contradictions when compared on genetic grounds. The pathogen is having a dramatic lethal effect on the natural populations of Platanus orientalis kashmeriana in Northern India and solid containment measures should be imposed to restrict its spread throughout the natural range of this ecologically, economically and historically precious host.
During the summer 2014, dark black symptoms beneath the leaf surfaces were observed on the Populas alba L species in the south Kashmir. In the later stages of development the infection subtends the whole surface resulting in leaf collapse. Fungal isolates were recovered directly from the structures present on the lesions. Purified DNA from each isolate was amplified using the random amplified polymorphic DNA technique recruiting the ITSI (TCCGTAGGTGAACCTGCGG) and ITS4 (TCCTCCGC TTATTGATATGC) specific primers. Amplification products visualized on agarose gel showed specific band pattern for each isolate. The mycotaxonomic characterization and phylogenetic interpretations showed the emergence of fungal species, Meliola sp. KY623717 with genetic variance of 2% in internal transcribed spacer (ITS) region of rDNA from closely related species under geographically distinct region.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.