First report of white stain of apple caused by <i>Trichothecium kashmeriana</i> in India

Dar, Rafiq Ahmad; Nand, Akhila; Shiekh, Imtiyaz Ahmad

doi:10.1080/03235408.2016.1264178

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…For phylogenetic analysis, ITS rDNA sequences from additional species were retrieved from GenBank. The phylogenetic tree based on concatenated ITS regions of rDNA strict consensus sequences of the Meliola mangiferae_ KY623717 and other fungal lineages used as out groups [5,6]. Consensus regions were compared against GenBank database using Mega BLAST program.…”

Section: Genotypic Observationsmentioning

confidence: 99%

Phylogeny of Meliola mangiferae Causing Black Mildews of Populas alba

Dar¹,

Akhila²,

Rai³

2017

Med Mycol Open Access

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During the summer 2014, dark black symptoms beneath the leaf surfaces were observed on the Populas alba L species in the south Kashmir. In the later stages of development the infection subtends the whole surface resulting in leaf collapse. Fungal isolates were recovered directly from the structures present on the lesions. Purified DNA from each isolate was amplified using the random amplified polymorphic DNA technique recruiting the ITSI (TCCGTAGGTGAACCTGCGG) and ITS4 (TCCTCCGC TTATTGATATGC) specific primers. Amplification products visualized on agarose gel showed specific band pattern for each isolate. The mycotaxonomic characterization and phylogenetic interpretations showed the emergence of fungal species, Meliola sp. KY623717 with genetic variance of 2% in internal transcribed spacer (ITS) region of rDNA from closely related species under geographically distinct region.

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Section: Genotypic Observationsmentioning

confidence: 99%

Phylogeny of Meliola mangiferae Causing Black Mildews of Populas alba

Dar¹,

Akhila²,

Rai³

2017

Med Mycol Open Access

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“…Both infected leaf/fruit bits were inoculated directly on to the PDA growth slants and incubated at 26 ∘ C for 15 days. Pure homogenous cultures were obtained after a number of successful subculturing attempts (Dar et al 2016) (deposited in University Phytopathology Unit with accession no. SU1099).…”

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confidence: 99%

Stigmina carpophila detected on Prunus armeniaca and Prunus persica in India

Dar

Nand

Shiekh

2017

Australasian Plant Dis. Notes

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During the late summer of 2016, a pattern of infection spots were observed in different varieties of Prunus armeniaca (Apricot) and Prunus persica (Peaches) in different locations in India. The disease led to a decline in the fruit production by almost 30% in the region. Symptomatologically, the dark brown leaf spots were hologenous, scattered over the entire surface, rounded to irregular, enlarging and coalescing, which resulted in premature defoliation. Both phenotypic & genotypic observations were carried out by double beam Scanning Electron Microscope (SEM) and Internal Transcribed Spacer (ITS) rDNA sequencing, respectively. To our knowledge it is the first report of this disease from India and solid containment measures should be imposed to restrict its spread throughout the natural range of these ecologically, economically and historically precious hosts.

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