A preliminary study on serum liver function indices of Diethylnitrosamine induced hepatocarcinogenesis and chemoprotective potential of in male Wistar rats , 7( 6): 439-442.
Eclipta albaVeterinary World
Porcine circovirus disease (PCVD), an emerging viral disease of economic importance, represents several disease manifestations principally caused by porcine circovirus 2 (PCV2). Prevalence of distinct PCV2 genotypes in pig population necessitates to study their replication potential in cell line in order to establish and compare infectivity and pathogenecity. Virus isolation forms an important aid in PCVD diagnosis, especially when multifactorial origin of disease condition is suspected. In the present study, isolation of PCV2 was accomplished by serially passaging the inoculum prepared from suspected tissue samples up to fourth passage in PK-15 cell line. Further, the confirmation of replication in cell line was done by immunoperoxidase monolayer assay and polymerase chain reaction. Information regarding virus isolation and identification of PCV2 isolates circulating in Indian pigs is scarce. In the present work, PCV2 was successfully isolated in PK-15 cell line and demonstrated their replication in cytoplasm and in nucleus by immunoperoxidase monolayer assay. Thus, virus isolation together with PCR assay helped to establish the PCV2 etiology in pneumonia and wasting of pigs encountered in the present study.All copyrights reserved to Nexus® academic publishers
Porcine reproductive and respiratory syndrome (PRRS) caused by an arterivirus is characterised by reproductive disorders in sows, and post-weaning pneumonia and growth reduction in piglets. Though the virus has been detected in Kerala, no systematic study has been carried out to ascertain its genotype and molecular epidemiology. In the present study, 7 PRRS virus (PRRSV) positive samples collected from incidences of PRRS in Kerala during 2017-2019 were subjected to ORF5, ORF7 and Nsp2 gene based reverse transcription polymerase chain reaction and the specific amplicons generated were sequenced. On BLAST analysis it was revealed that all the sequences were of genotype 2 (North American genotype). Phylogenetic analysis of ORF5 sequences, grouped them under subgenotype 4 with close clustering with other isolates from Kerala, Mizoram and Assam. Nsp2 gene sequence based phylogenetic analysis grouped the isolates under subgenotype 3 with similarities to isolates from Mizoram. Phylogenetic analysis based on ORF7, clustered the isolates under study with PRRSV isolates from Mizoram and Meghalaya. In Nsp2 sequences, a 30 amino acid discontinuous deletion was observed. On analysis of amino acid sequences of ORF5 of Kerala isolates and those from India, it was seen that the Kerala isolates showed closer similarity to PRRSV isolates from Assam than to the other Indian isolates. The study reveals that PRRSV strains prevalent in Kerala share close relationship with other PRRSV isolates in India. This may be due to spread of the virus from these regions to Kerala due to animal movement. Concerted efforts should be undertaken to check unauthorized animal movement to control spread of this economically important disease.
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