A preliminary study on serum liver function indices of Diethylnitrosamine induced hepatocarcinogenesis and chemoprotective potential of Eclipta alba in male Wistar rats

Anoopraj, R.; Hemalatha, S; Balachandran, C.

doi:10.14202/vetworld.2014.439-442

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…ROS play an important role in different types of cancer development [24]. Furthermore, NDEA increased oxidative stress through hepatic metabolism via the cytochrome p450 enzyme [25]. Ethyldiazonium ion alkylates nuclear DNA bases through NDEA to form adducts such as O4 and O6ethyldeoxythymidine and O6-ethyldeoxyguanosine [26].…”

Section: Discussionmentioning

confidence: 99%

Involvement of PI3K/HIF-1α/c-MYC/iNOS Pathway in the Anticancer Effect of Suaeda vermiculata in Rats

Mohammed,

Ewees,

Mahmoud

et al. 2023

Pharmaceuticals

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Suaeda vermiculata Forssk. ex JF Gmel. (SV), a traditional known plant, has shown in vitro cytotoxic activity against HepG2 and HepG-2/ADR (doxorubicin-resistant cells) liver cell carcinoma cell lines, as well as hepatoprotection against paracetamol and carbon tetrachloride (CCl4)-induced liver injury. The current study evaluated the protective effect of SV, administered against N-diethylnitrosamine (NDEA)-induced HCC in rats. The possible modulatory effect of SV on the PI3K/HIF-1α/c-MYC/iNOS pathway was investigated. Sixty male adult albino rats (200 ± 10 g) were equally classified into five groups. Group I served as a control; Group 2 (SV control group) received SV (p.o., 200 mg/kg body weight); Group 3 (NDEA-administered rats) received freshly prepared NDEA solution (100 mg/L); and Groups 4 and 5 received simultaneously, for 16 weeks, NDEA + SV extract (100 and 200 mg/kg, orally). NDEA-treated rats displayed significant increases in serum levels of AFP, CEA, PI3K, malondialdehyde (MDA), epidermal growth factor receptor (EGFR), and vascular endothelial growth factor (VEGFR), with increased liver tissue protein expression of fibrinogen concomitant and significantly decreased concentrations of antioxidant parameters (catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH)) in comparison to normal rats. On the flip side, AFP, CEA, PI3K, MDA, EGFR, and VEGFR serum levels were significantly reduced in rats that received NDEA with SV, both at low (SV LD) and high (SV HD) doses, accompanied by significant improvements in antioxidant parameters compared to the NDEA-treated group. Conclusions: SV possesses a significant hepatoprotective effect against NDEA-induced HCC via inhibiting the PI3K/HIF-1α/c-MYC/iNOS pathway, suggesting that SV could be a promising hepatocellular carcinoma treatment.

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Section: Discussionmentioning

confidence: 99%

Involvement of PI3K/HIF-1α/c-MYC/iNOS Pathway in the Anticancer Effect of Suaeda vermiculata in Rats

Mohammed,

Ewees,

Mahmoud

et al. 2023

Pharmaceuticals

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“…Elevation of serum ALT, AST, and ALP activity indicates the abnormality of liver function [24]. And the increase of creatinine concentration in serum results in renal injury [25].…”

Section: Discussionmentioning

confidence: 99%

Effects of Astragalus membranaceus roots supplementation on growth performance, serum antioxidant and immune response in finishing lambs

Hao

Wang

Ren

et al. 2020

Asian-Australas J Anim Sci

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Objective: Astragalus membranaceus root is a well-known traditional Chinese herbal medicine with many biological active constituents. This study was conducted to examine the effects of Astragalus membranaceus root powder (AMP) on growth performance, serum antioxidant and immune response in finishing lambs.Methods: A total of thirty-six Guangling fat-tailed ram lambs (body weight = 19±2 kg, mean ±standard deviation) were randomly assigned to one of six treatments for a 40 d feeding period, with the first 10 d for adaptation. Treatments consisted of the lambs’ basal diets with addition of 0, 5, 10, 15, 20, and 30 g/kg of diet of AMP.Results: Response to supplementation level of AMP was quadratic (p≤0.032) for final weight and ADG with the greatest at 10 g/kg of diet, but dry matter intake was not affected (p≥0.227) by treatments. The increase of AMP supplementation resulted in a quadratic response in contents of triglyceride and creatinine (p<0.05), with the lowest values for 10 and 20 g/kg of diet, respectively. A linear and quadratic decrease was observed in activity of alkaline phosphatase in serum of lambs. As the AMP supplementation increased, the activities of total superoxide dismutase and total antioxidant capacity increased linearly (p≤0.018) and hydroxyl radical (OH–) decreased linearly (p = 0.002). For catalase (CAT) and malondialdehyde (MDA), quadratic (p≤0.001) effects were observed among treatments, with the greatest CAT and lowest MDA values at 10 g/kg AMP. Additionally, supplementing AMP up to a level of 10 or 15 g/kg of diet quadratically increased immunoglobulin and interleukin contents in the serum.Conclusion: The results indicated that AMP can be used as natural feed additive in the ration of lambs to improve ADG, antioxidant status, and immune functions, and the optimal dose was 10 g/kg of diet under the condition of this experiment.

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“…Various investigations have shown that toll-like receptors (TLRs) and their pro-inflammatory mediators may be of the host inflammatory response to infection and play a significant role in human hepatic disease. 23,36 Diethylnitramine (nitroso family compound), a wellknown hepato-carcinogenic agent, can be contained in water, tobacco smoke, agricultural chemicals, fried meals, cured meals, cheddar cheese, pharmaceutical and cosmetic products, and in trace amounts in baby bottle nipples. Studies suggest that the DEN-induced hepatocellular carcinoma in the rodents was similar to human hepatocellular carcinoma.…”

Section: Dovepressmentioning

confidence: 99%

“…34,41 Previous studies suggest that DEN was metabolized in hepatic tissue via the action of cytochrome p450 enzymes and finally increased oxidative stress. 23,36,42 During the metabolism in hepatic tissue and reactive with the various metabolites, these are mainly responsible for the hepatic toxic effects. DEN bioactivates the ethyldiazonium ion, which alkylates DNA bases to form promutagenic adducts like O6-ethyldeoxyguanosine and O4 and O6ethyldeoxythymidine.…”

Section: Dovepressmentioning

confidence: 99%

“…Antioxidants play a significant role in scavenging the tissue and cells, thereby protecting cellular and tissue damage from disease. 36,49 Umbelliferone, a coumarin derivative mainly found in Agele marmilosa, is well known antioxidant enzymes. Kumar et al showed that the DEN administration boosted the lipid peroxidation reaction along with the suppression of endogenous antioxidant enzymes.…”

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confidence: 99%

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Curcumae Ameliorates Diethylnitrosamine-Induced Hepatocellular Carcinoma via Alteration of Oxidative Stress, Inflammation and Gut Microbiota

Zhang¹,

Li²,

Li³

2021

JIR

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Background: Nonalcoholic fatty liver disease (NAFLD) increased the risk factor of hepatocellular carcinoma (HCC). NAFLD induces the hepatic-related cancer deaths mostly in middle-aged men. NAFLD enhanced the inflammatory reaction and oxidative stress in the hepatic tissue. Curcumae exhibited the anti-inflammatory and antioxidant effects. In this study, we made an attempt to scrutinize the protective effect of curcumae on obesity-induced HCC via alteration of inflammation, oxidative stress and gut microbiota. Methods: The rats used in this experiment were Wistar rats, 100 mg/kg intraperitoneal injection of diethylnitrosamine (hepatic carcinogen) was used at 2 weeks. After 6 weeks of the experimental study, the rats were randomly divided into high-fat diet (HFD) with or without curcumae-treated group rats and received the treatment for 22 weeks. Hepatic, nonhepatic, cardiac, antioxidant, pro-inflammatory and inflammatory were estimated at the end of the study. The stools of the experimental rats were collected for estimating the gut microbiota. Results: Curcumae-treated group rats exposed reduction of the hepatic nodules in hepatic tissue. Curcumae significantly (P<0.001) diminished the level of hepatic parameters and antioxidant parameters in the serum. Curcumae significantly (P<0.001) suppressed the proinflammatory cytokines level, viz. interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), interleukin-7 (IL-7) and augmented the level of interleukin-10 (IL-10) in the serum and hepatic tissue. Curcumae significantly (P<0.001) suppressed inflammatory mediators including cyclooxygenase (COX-2), prostaglandin E2 (PGE2) and nuclear factor kappa B (NF-κB) in the serum and hepatic tissue. Furthermore, curcumae increased the gut microbial diversity and richness and decreased the relative abundance of genus Mucispirillum and Clostridium, respectively. Conclusion: Curcumae prevents HFD-induced inflammation during the hepatic carcinoma by modulating the oxidative stress, inflammatory reaction and gut microbiota.

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A preliminary study on serum liver function indices of Diethylnitrosamine induced hepatocarcinogenesis and chemoprotective potential of Eclipta alba in male Wistar rats

Abstract: A preliminary study on serum liver function indices of Diethylnitrosamine induced hepatocarcinogenesis and chemoprotective potential of in male Wistar rats , 7( 6): 439-442. Eclipta albaVeterinary World

Cited by 6 publications

References 11 publications

Involvement of PI3K/HIF-1α/c-MYC/iNOS Pathway in the Anticancer Effect of Suaeda vermiculata in Rats

Involvement of PI3K/HIF-1α/c-MYC/iNOS Pathway in the Anticancer Effect of Suaeda vermiculata in Rats

Effects of Astragalus membranaceus roots supplementation on growth performance, serum antioxidant and immune response in finishing lambs

Curcumae Ameliorates Diethylnitrosamine-Induced Hepatocellular Carcinoma via Alteration of Oxidative Stress, Inflammation and Gut Microbiota

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