Identification and genetic analysis of infectious bursal disease viruses from field outbreaks in Kerala, India

Nandhakumar, D.; Rajasekhar, R.; Logeshwaran, G; Ravishankar, Chintu; Sebastian, Stephy Rose; Anoopraj, R.; Sumod, K.; Mani, Binu K.; Chaithra, G; Deorao, Chandankar Vaidehi; John, Koshy

doi:10.1007/s11250-019-02084-w

Cited by 7 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the vaccinated RT-PCR is said to be most sensitive method of diagnosis (Jackwood et al 1997;Van den Berg, 2000). In the recent reports, approximate level of prevalence were recorded by Nandhakumar et al (2020) from India and Mawgod et al (2014) and Nwagbo et al(2016) from Africa and Zahoor et al (2011) from Pakistan, respectively, they recorded 53.65, 38.46 and 37.93 and 50.0 per cent of disease incidence using RT PCR. However, much higher rates as 73.33 and 85.0 per cent were revealed by Singh et al (2014) and Mittal et al (2005) from Madhya Pradesh and Haryana states of India.…”

Section: Resultsmentioning

confidence: 98%

“…Other tests that have been used are fluorescent antibody techniques, immunohistochemistry and molecular techniques. Currently, reverse transcriptase-polymerase chain reaction (RT-PCR) is the molecular tool which is specific and sensitive method for diagnosis of IBDV infection (Kataria et al 2001, Nandhakumar et al 2020. RT-PCR can be further used in comparison of IBDV strains, especially for detection of vvIBDV strains, by supplementation with restricted fragment length polymorphism (RFLP)/ restriction enzyme digestion (RE digestion) and nucleic and amino acid sequencing methods (Jackwood et al2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular and Cultures based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Vaccinated and Non Vaccinated Poultry Flocks

Shinde

Chauhan

Patel

et al. 2021

IJAR

View full text Add to dashboard Cite

Background: In recent years, Infectious bursal disease is continuously occurring even after vaccination in India and requires an inclusive diagnosis. Therefore, the present study was undertaken to diagnose IBD through molecular and culture methods. Methods: One pooled sample, from each of 54 flocks having birds with IBD like symptoms, was collected. History of bird type, age and vaccination was recorded. Samples were subjected to RT-PCR, egg embryo culture and chicken fibroblast cells culture. Result: A total of 49669 out of 517900 (9.59 %) of birds, aging 3-6 weeks, were displaying the signs similar to IBD.In RT-PCR, 21 (38.88%) samples were found positive which belonged to11 (52.38%) vaccinated and 10 (47.62%) unvaccinated flocks.The RT-PCR positive samples were successfully cultivated for the virus through egg embryo and cell culture. The CEF culture was found least sensitive compared to egg embryo culture and RT-PCR.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Molecular and Cultures based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Vaccinated and Non Vaccinated Poultry Flocks

Shinde

Chauhan

Patel

et al. 2021

IJAR

View full text Add to dashboard Cite

show abstract

“…This study reveals the close relation of IBD/CVAS/6 with intermediate plus vaccine virus as the sample was obtained from a vaccinated flock. The field isolates showing nucleotide and amino acid similarity wth intermediate plus vaccine strains were reported by Deorao et al, (2021) and Nandhakumar et al (2020). The authors opined that the use of intermediate plus vaccine may induce bursal lesions and immunosuppression in the flock.…”

Section: Sequencing and Phylogenetic Analysis Of Vp3 Genementioning

confidence: 96%

Sequence analysis of VP1, VP2 and VP3 genes of Infectious Bursal Disease Virus from a field outbreak in Kerala, India

Joy¹,

Nair²,

Ambily³

et al. 2022

Journal of Veterinary and Animal Sciences

View full text Add to dashboard Cite

Infectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is one among the top five infectious diseases of poultry that discernibly affects commercial poultry industry. The mutating viral genome of IBDV accounts for disease outbreaks in fields even after following stringent biosecurity measures and vaccination protocols. The present study is focussed on the characterisation of an IBDV field virus, IBD/CVAS/6, from a vaccinated flock in Kerala, based on the sequence analysis of VP1, VP2 and VP3 genes. Bursa of Fabricius samples collected from a 26 days-old chicken flock from a suspected outbreak in the Thiruvananthapuram district of Kerala formed the subject of the study. Reverse transcriptase-polymerase chain reaction (RT-PCR) targeting VP2 gene confirmed the presence of virus in the sample. The sequence analysis revealed that the deduced amino acid sequence of VP1 gene of IBD/CVAS/6 was 100 per cent homologous with an attenuated very virulent vaccine strain of Israel, mb and the VP2 gene was 100 per cent homologous with mb and Ventri IBDV plus vaccine strain of India. The analysis of VP3 gene also revealed the similarity with vaccine strains except for a single variation S745N in its deduced amino acid sequence. The phylogenetic analysis of IBD/CVAS/6 revealed it’s close relation with mb, Ventri IBDV plus and a very virulent strain of Israel, ks. The characteristic virulent marker amino acid motifs ‘SWSASGS’ and ‘TDN’ were present in the VP2 and VP1 genes, respectively. Hence, the study revealed that the obtained virus has emerged from an attenuated very virulent vaccine strain and hence the present study is a report of involvement of the intermediate plus vaccine strain in field outbreaks in Kerala. The role of S745N in virulence cannot be accounted from the present study, however the involvement of IBD/CVAS/6 in the outbreak might be related to S745N variation or variations in other genes of the virus or due to the inefficiency of the vaccine or vaccination protocol followed, which can be defined only after further studies. The present report is the first characterisation study in Kerala focussing on the analysis of VP1, VP2 and VP3 genes of IBDV.

show abstract

“…The leaf of Moringa oleifera is dorsiventral, meaning that it has two distinct sides: an upper adaxial surface and a lower abaxial surface [38] . The adaxial surface is covered by a waxy cuticle layer and has a layer of epidermal cells with stomata, which are specialized pores that allow for gas exchange [39][40] .…”

Section: Leafmentioning

confidence: 99%

An exhaustive review on pharmacological potential, bioactive components and formulations of Moringa oleifera

Dimple Chauhan,

Archana,

Abhishek Yadav

2024

World J. Adv. Res. Rev.

View full text Add to dashboard Cite

Moringa oleifera, native to India, grows in the tropical and subtropical regions of the world. It is commonly known as ‘drumstick tree’ or ‘horseradish tree’ and has gained attention in recent years for its nutritional and medicinal properties. This review article aims to provide a comprehensive overview of the current scientific understanding of Moringa oleifera. The review covers the plant's botany, nutritional composition, and phytochemical content. It also discusses the plant's pharmacological and medicinal properties, including its anti-inflammatory, anti-cancer, anti-diabetic, anti-viral and anti-microbial activities. Additionally, the review examines the potential applications of Moringa oleifera in food and agriculture, as well as its industrial uses, such as in biofuel production. Summarizing, this review article provides a comprehensive and scientific overview of Moringa oleifera, highlighting its potential as a valuable plant resource with numerous applications and benefits for human health and well-being. The review article also identifies areas for future research to further explore the plant's potential and unlock its full potential as a natural resource.

show abstract

Identification and genetic analysis of infectious bursal disease viruses from field outbreaks in Kerala, India

Cited by 7 publications

References 42 publications

Molecular and Cultures based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Vaccinated and Non Vaccinated Poultry Flocks

Molecular and Cultures based Diagnosis of Infectious Bursal Disease Virus (IBDV) in Vaccinated and Non Vaccinated Poultry Flocks

Sequence analysis of VP1, VP2 and VP3 genes of Infectious Bursal Disease Virus from a field outbreak in Kerala, India

An exhaustive review on pharmacological potential, bioactive components and formulations of Moringa oleifera

Contact Info

Product

Resources

About