No abstract

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

No abstract

No abstract

The present study highlighted that the synthesis of β-Glucan nanoparticles (β-GluNPs) developed as a facile method to prevent cancer and infectious diseases, which is highly effective and inexpensive in the biological field. This research study has demonstrated the use of marine algae (Gracilaria corticate) to extract water-soluble β-Glucan and synthesis of β-GluNPs by dissolving the extracted β-Glucan in NaOH under optimal conditions. The molecular structure of the extracted and stabilized β-GluNPs was analysed using NMR. Further, the Physico-chemical parameters of β-GluNPs were analysed by the high throughput instruments like UV spectroscopy, FTIR, DLS, ZETA Potential, SEM, HRTEM, and XRD analysis. The synthesis of β-GluNPs confirmed by IR spectroscopy. The HR-TEM results demonstrated that the formation of polydispersed nanoparticles with a mean size of 20 ± 5 nm. The hydrostatic zeta potential was − 22.7mV, which indicated their colloidal stability. The XRD pattern revealed the crystalline nature of the nanoparticles. Besides, β-GluNPs performed better antibacterial activity against the tested pathogens. The apoptosis and DNA fragmentation observed to be IC50 42.5 µg/ml of the β-GluNPs. The DNA fragmentation assay indicated the selective inhibition of the MCF-7 cell line by DNA damage. Hence, β-GluNPs used as a promising alternative drug against human breast cancer.